Cell Culture Products
Welcome to Cytion, your destination for cutting-edge cell culture solutions. Our portfolio spans high-quality human cell lines, animal cell lines, and primary cells designed to bridge the gap between innovative research and practical application. Each of our offerings, from our carefully isolated primary cells to our versatile line-derived DNA, is crafted to enhance your cell culture workflows and ensure reproducible results. Discover our specialized cell culture media as well, tailored to meet the needs of your cells for optimal growth and vitality. Dive into the world of Cytion, where quality and innovation come together seamlessly.
Human cell lines offer valuable insights into disease mechanisms and play a pivotal role in the production of therapeutic proteins, antibodies, and in the advancement of vaccine development. We specialize in supplying authenticated and contamination-free human cell lines to support scientific and medical breakthroughs.
Explore our high-quality animal cell lines, perfect for researchers seeking reliable results. Our cell bank offers a diverse selection of authenticated, contamination-free animal cell lines to support your scientific endeavors. Advance your research with our trusted cellular resources, available for immediate global dispatch.
Discover our collection of stem cells, designed to meet the demands of cutting-edge research in regenerative medicine and drug discovery. Our stem cells are ethically sourced and characterized to empower your scientific breakthroughs. Elevate your research with our high-quality stem cells.
Our primary cells, directly isolated from healthy tissues, to provide you with physiologically relevant models for biomedical research. Cells are verified to retain their biological properties, ensuring authentic responses in your experiments. Choose our primary cells for a reliable foundation in your research.
Our Quality Promise
Every batch of our cell lines—including human, hamster, mouse, rat, and dog cells—is rigorously authenticated through Short Tandem Repeat (STR) analysis to ensure genetic consistency and reliability. Should you need a comprehensive, publication-grade STR report for your cells, please feel free to reach out to us.
To ensure the purity of our cell lines, we conduct extensive testing for contaminants. For mycoplasma detection, we employ sensitive PCR-based and luminescence-based assays. Moreover, our comprehensive viral panels rigorously screen for viral contaminants, while our standardized manufacturing processes systematically check for any bacterial or fungal presence.
Our cell lines closely match their genetic origins, thanks to extensive validation processes. We maintain the integrity of the cells by limiting propagation to just 3-4 passages when generating new cell stocks, thus providing you with cell cultures that are as close to the genetic origin as possible.
Researchers' Favorites
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.
Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:
8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)
This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.
Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Salts
Potassium chloride
200
Potassium phosphate monobasic anhydrous
200
Sodium chloride
8000
Sodium phosphate dibasic anhydrous
1150
This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.
DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.
To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.
DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +8°C, protected from light.
Once opened, store at 4°C and use within 6–8 weeks.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Amino Acids
Glycine
18.75
L-Alanine
4.45
L-Arginine HCl
147.50
L-Asparagine H₂O
7.50
L-Aspartic Acid
6.65
L-Cysteine HCl H₂O
17.56
L-Cystine 2 HCl
31.29
L-Glutamic Acid
7.35
L-Glutamine
365.00
L-Histidine HCl H₂O
31.48
L-Isoleucine
54.47
L-Leucine
59.05
L-Lysine HCl
91.25
L-Methionine
17.24
L-Phenylalanine
35.48
L-Proline
17.25
L-Serine
26.25
L-Threonine
53.45
L-Tryptophan
9.02
L-Tyrosine Disodium Salt
48.10
L-Valine
52.85
Vitamins
D-Biotin
0.0035
Choline Chloride
8.98
D-Calcium Pantothenate
2.24
Folic Acid
2.66
myo-Inositol
12.60
Nicotinamide
2.02
Pyridoxine HCl
0.031
Pyridoxal HCl
2.00
Riboflavin
0.219
Thiamine HCl
2.17
Vitamin B12
0.68
Inorganic Salts
CaCl₂ 2 H₂O
154.50
CuSO₄ 5 H₂O
0.0013
Fe(NO₃)₃ 9 H₂O
0.05
FeSO₄ 7 H₂O
0.417
KCl
311.80
MgCl₂ 6 H₂O
61.20
MgSO₄
48.84
NaCl
6996.00
NaHCO₃
1200.00
Na₂HPO₄
71.02
NaH₂PO₄
54.30
ZnSO₄ 7 H₂O
0.432
Other Components
D-Glucose
3151.00
Hypoxanthine
2.40
HEPES
3574.50
Linoleic Acid
0.042
Lipoic Acid
0.105
Phenol Red Sodium Salt
8.63
Putrescine 2 HCl
0.081
Sodium Pyruvate
55.00
Thymidine
0.365
- A Gentle Alternative to Trypsin
Accutase is a cell detachment solution that is revolutionizing the cell culture industry. It is a mix of proteolytic and collagenolytic enzymes that mimics the action of trypsin and collagenase. Unlike trypsin, Accutase does not contain any mammalian or bacterial components and is much gentler on cells, making it an ideal solution for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. In this blog post, we will explore the benefits and uses of Accutase and how it is changing the game in cell culture.
Advantages of Accutase
Accutase has several advantages over traditional trypsin solutions. Firstly, it can be used whenever gentle and efficient detachment of any adherent cell line is needed, making it a direct replacement for trypsin. Secondly, Accutase works extremely well on embryonic and neuronal stem cells, and it has been shown to maintain the viability of these cells after passaging. Thirdly, Accutase preserves most epitopes for subsequent flow cytometry analysis, making it ideal for cell surface marker analysis.
Additionally, Accutase does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. This eliminates the need for an inactivation step and saves time for cell culture technicians. Finally, Accutase does not need to be aliquoted, and a bottle is stable in the refrigerator for 2 months.
Applications of Accutase
Accutase is a direct replacement for trypsin solution and can be used for the passaging of cell lines. Additionally, Accutase performs well when detaching cells for the analysis of many cell surface markers using flow cytometry and for cell sorting. Other downstream applications of Accutase treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Composition of Accutase
Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. It is formulated at a much lower concentration than trypsin and collagenase, making it less toxic and gentler, but just as effective.
Efficiency of Accutase
Accutase has been shown to be efficient in detaching primary and stem cells and maintaining high cell viability compared to animal origin enzymes such as trypsin. 100% of cells are recovered after 10 minutes, and there is no harm in leaving cells in Accutase for up to 45 minutes, thanks to autodigestion of Accutase.
In summary
In conclusion, Accutase is a powerful solution that is changing the game in cell culture. With its gentle nature, efficiency, and versatility, Accutase is the ideal alternative to trypsin. If you are looking for a reliable and efficient solution for cell detachment, Accutase is the solution for you.
What sets DMEM apart from other media is its unique composition. It contains an impressive fourfold increase in amino acid and vitamin concentration compared to the original Eagle's Minimal Essential Medium. Initially developed with low glucose (1 g/L) and sodium pyruvate, DMEM is frequently employed with higher glucose levels, either with or without sodium pyruvate. Notably, DMEM does not contain proteins, lipids, or growth factors, necessitating supplementation. To achieve optimal growth, a common approach is to supplement DMEM with 10% Fetal Bovine Serum (FBS). Additionally, DMEM employs a sodium bicarbonate buffer system, requiring a 5-10% CO2 environment to maintain a physiological pH.
Dulbecco's Modified Eagle Medium (DMEM) is highly regarded among defined media for cell and tissue culture, catering to the growth needs of various adherent cell phenotypes. It surpasses the original Eagle's Medium, developed in the 1950s for cultivating chicken cells, through the enhanced supplementary formulation known as Dulbecco's modification. This modification significantly elevates the content of select amino acids and vitamins up to fourfold compared to the original medium.
In the field of cell culture, DMEM plays a vital role as a growth medium for different cell types, including primary cells, stem cells, and transformed cells. Researchers also employ the modified version of DMEM for a wide array of research applications, such as drug discovery, tissue engineering, and the study of cell signaling pathways.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +8°C, protected from light.
Once opened, store at 4°C and use within 6–8 weeks.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Amino Acids
Glycine
30.00
L-Arginine HCl
84.00
L-Cystine 2 HCl
62.57
L-Glutamine
584.00
L-Histidine HCl H2O
42.00
L-Isoleucine
105.00
L-Leucine
105.00
L-Lysine HCl
146.00
L-Methionine
30.00
L-Phenylalanine
66.00
L-Serine
42.00
L-Threonine
95.00
L-Tryptophan
16.00
L-Tyrosine 2 Na 2H2O
103.79
L-Valine
94.00
Vitamins
Choline chloride
4.00
D-Calcium Pantothenate
4.00
Folic Acid
4.00
myo-Inositol
7.20
Nicotinamide
4.00
Pyridoxal HCl
4.00
Riboflavin
0.40
Thiamine HCl
4.00
Inorganic Salts
CaCl2 2 H2O
265.00
Fe(NO3)3 9 H2O
0.10
KCl
400.00
MgSO4 7H2O
200.10
NaCl
6400.00
NaHCO3
3700.00
NaH2PO4 2H2O
141.73
Other Components
D-Glucose
4500.00
Phenol Red Sodium Salt
15.90
Sodium Pyruvate
110.00
Discover the advanced NCI-H295R Cell Growth Medium, an expertly crafted nutrient solution specifically designed to foster the growth and maintenance of mammalian cell lines, including the specialized NCI-H295R cells. This medium stands out with its unique blend of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Nutrient Mixture F-12) in a precise 1:1 ratio, enriched with essential supplements to meet the intricate needs of cell culture.
Enhanced Formulation for Superior Cell Culture Performance
Supplemented Baseline: The basic medium is already rich, combining DMEM's high amino acid and vitamin concentrations with the diverse nutrients of Ham's F-12. To this potent mix, we've added 5% Fetal Bovine Serum (FBS), providing crucial growth factors, hormones, and proteins.
Tailored Additives: Beyond FBS, this medium is fortified with critical supplements including Insulin, Transferrin, Bovine Serum Albumin, Selenious Acid, and Lineloic Acid. These additions cater to specific cellular requirements, promoting enhanced cell growth, metabolic function, and overall health.
L-Glutamine Boost: The presence of L-glutamine further amplifies the medium's efficacy, supporting essential metabolic processes and encouraging robust cell proliferation.
Optimized for Cell Culture
pH Controlled Environment: Incorporating a bicarbonate buffer system and phenol red indicator, the medium ensures a stable pH environment. It is optimized for a 5% CO2 atmosphere, allowing for precise pH monitoring and adjustment.
Quality and Purity: Each lot of basic medium undergoes rigorous testing for sterility, mycoplasma, and bacteria, ensuring the medium's integrity. With a pH maintained medium, you can trust in the consistent quality and performance of this medium.
Handling and Storage
Maintain the medium's quality by storing it refrigerated at +2°C to +8°C, away from light. Avoid extremes, such as freezing or overheating, to preserve its efficacy. For partial use, gently warm the needed quantity at room temperature, ensuring the medium is brought to the appropriate condition without compromising its components.
Shelf life
With an 8-week shelf life from the manufacturing date, the NCI-H295R Cell Growth Medium is a reliable choice for your cell culture needs, ensuring the vitality and productivity of your research.