RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3
€25.00*
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
This RPMI 1640 medium contains 4.5 grams per liter of glucose.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium nitrate x 4H2O
100,00
Magnesium sulfate anhydrous
48,83
Potassium chloride
400,00
Sodium chloride
5450,00
di-Sodium hydrogen phosphate
800,49
Other Components
D(+)-Glucose anhydrous
4500,00
Glutathione (red.)
1,00
HEPES
2383,00
Phenol red
5,00
Sodium pyruvate
110,00
Amino Acids
L-Arginine x HCl
241,86
L-Asparagine x H2O
56,82
L-Aspartic acid
20,00
L-Cystine x 2HCl
65,19
L-Glutamine
300,00
L-Glutamic acid
20,00
Glycine
10,00
L-Histidine x HCl x H2O
20,27
L-Hydroxyproline
20,00
L-Isoleucine
50,00
L-Leucine
50,00
L-Lysine x HCl
40,00
L-Methionine
15,00
L-Phenylalanine
15,00
L-Proline
20,00
L-Serine
30,00
L-Threonine
20,00
L-Tryptophan
5,00
L-Tyrosine x 2Na
28,83
L-Valine
20,00
Vitamins
p-Aminobenzoic acid
1,00
D-(+)-Biotin
0,20
D-Calcium pantothenate
0,25
Choline chloride
3,00
Folic acid
1,00
myo-Inositol
35,00
Nicotinamide
1,00
Pyridoxine x HCl
1,00
Riboflavin
0,20
Thiamine x HCl
1,00
Vitamin B12
0,01
NaHCO3
1500,00
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
This RPMI 1640 medium contains 4.5 grams per liter of glucose.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium nitrate x 4H2O
100,00
Magnesium sulfate anhydrous
48,83
Potassium chloride
400,00
Sodium chloride
5450,00
di-Sodium hydrogen phosphate
800,49
Other Components
D(+)-Glucose anhydrous
4500,00
Glutathione (red.)
1,00
HEPES
2383,00
Phenol red
5,00
Sodium pyruvate
110,00
Amino Acids
L-Arginine x HCl
241,86
L-Asparagine x H2O
56,82
L-Aspartic acid
20,00
L-Cystine x 2HCl
65,19
L-Glutamine
300,00
L-Glutamic acid
20,00
Glycine
10,00
L-Histidine x HCl x H2O
20,27
L-Hydroxyproline
20,00
L-Isoleucine
50,00
L-Leucine
50,00
L-Lysine x HCl
40,00
L-Methionine
15,00
L-Phenylalanine
15,00
L-Proline
20,00
L-Serine
30,00
L-Threonine
20,00
L-Tryptophan
5,00
L-Tyrosine x 2Na
28,83
L-Valine
20,00
Vitamins
p-Aminobenzoic acid
1,00
D-(+)-Biotin
0,20
D-Calcium pantothenate
0,25
Choline chloride
3,00
Folic acid
1,00
myo-Inositol
35,00
Nicotinamide
1,00
Pyridoxine x HCl
1,00
Riboflavin
0,20
Thiamine x HCl
1,00
Vitamin B12
0,01
NaHCO3
1500,00
EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA
€25.00*
One of the most widely used synthetic cell culture media is Minimum Essential Medium Eagle (MEM). Developed by Harry Eagle, this medium was first introduced in 1959 and has since become a popular choice for various cell types grown in monolayers and adherent cell lines.
This EMEM medium consists of 2 mM L-Glutamine, 1.5 g/L NaHCO3, EBSS, 1 mM Sodium pyruvate, and NEAA.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, L-glutamine, sodium pyruvate, and sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, storing the medium at two °C to 8°C in the dark when not in use is recommended.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
264,92
Magnesium sulfate
97,67
Potassium chloride
400,00
Sodium chloride
6,800.00
Sodium dihydrogen phosphate x H2O
140,00
Other Components
D(+)-Glucose
1,000.00
Phenol red
10,00
Sodium pyruvate
110,00
NaHCO3
1,500.00
Amino Acids
L-Alanine
8,90
L-Arginine x HCl
126,00
L-Asparagine x H2O
13,20
L-Aspartic acid
13,30
L-Cystine
24,00
L-Glutamine
292,30
L-Glutamic acid
14,70
Glycine
7,50
L-Histidine x HCl x H2O
42,00
L-Isoleucine
52,00
L-Leucine
52,00
L-Lysine x HCl
72,50
L-Methionine
15,00
L-Phenylalanine
32,00
L-Proline
11,50
L-Serine
10,50
L-Threonine
48,00
L-Tryptophan
10,00
L-Tyrosine
36,00
L-Valine
46,00
Vitamins
D-Calcium pantothenate
1,00
Choline chloride
1,00
Folic acid
1,00
myo-Inositol
2,00
Nicotinamide
1,00
Pyridoxal x HCl
1,00
Riboflavin
0,10
Thiamine x HCl
1,00
This EMEM medium consists of 2 mM L-Glutamine, 1.5 g/L NaHCO3, EBSS, 1 mM Sodium pyruvate, and NEAA.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, L-glutamine, sodium pyruvate, and sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, storing the medium at two °C to 8°C in the dark when not in use is recommended.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
264,92
Magnesium sulfate
97,67
Potassium chloride
400,00
Sodium chloride
6,800.00
Sodium dihydrogen phosphate x H2O
140,00
Other Components
D(+)-Glucose
1,000.00
Phenol red
10,00
Sodium pyruvate
110,00
NaHCO3
1,500.00
Amino Acids
L-Alanine
8,90
L-Arginine x HCl
126,00
L-Asparagine x H2O
13,20
L-Aspartic acid
13,30
L-Cystine
24,00
L-Glutamine
292,30
L-Glutamic acid
14,70
Glycine
7,50
L-Histidine x HCl x H2O
42,00
L-Isoleucine
52,00
L-Leucine
52,00
L-Lysine x HCl
72,50
L-Methionine
15,00
L-Phenylalanine
32,00
L-Proline
11,50
L-Serine
10,50
L-Threonine
48,00
L-Tryptophan
10,00
L-Tyrosine
36,00
L-Valine
46,00
Vitamins
D-Calcium pantothenate
1,00
Choline chloride
1,00
Folic acid
1,00
myo-Inositol
2,00
Nicotinamide
1,00
Pyridoxal x HCl
1,00
Riboflavin
0,10
Thiamine x HCl
1,00
Freeze medium CM-ACF - serum free - 50 ml
€59.00*
Cryopreservation Media Variants:
50 ml
Cytion’s Freeze Medium CM-ACF is an advanced serum-free cryopreservation medium tailored to ensure exceptional cell viability and functionality post-thaw. This versatile medium is suitable for a wide range of cell types, including both human and animal cells, making it an indispensable tool for diverse research applications. Formulated with a carefully balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-ACF minimizes ice crystal formation and cellular stress during the freezing process, thereby preserving cellular integrity.
Key Features of Freeze Medium CM-ACF
Serum-Free Formulation: Eliminates the variability and risks associated with serum, providing a defined and controlled environment for a wide array of cell types, including primary cells, stem cells, and established cell lines.
Broad Compatibility: Effective for a wide array of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-ACF is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Using CM-ACF for Freezing Cells
To use CM-ACF for freezing both adherent and suspension cells, follow these steps:
Preparation:
For adherent cells: Wash and dissociate them from the culture substrate.
For suspension cells: Proceed directly to the next step.
Cell Counting: Ensure cells are at the proper concentration.
Centrifugation: Pellet the cells and resuspend them in CM-ACF freeze medium.
Cryovial Transfer: Transfer the resuspended cells into cryovials.
Freezing Process: Use a slow-freezing method before transferring the cells to long-term storage.
Freezing Methods
Method
Description
Steps
❄️ Manual Freezing
A step-by-step method involving gradual temperature reduction to ensure cell viability.
1. Place cells in freeze medium in a 4°C freezer for 40 minutes. 2. Transfer to a -80°C freezer for 24 hours. 3. Store cells in liquid nitrogen for long-term preservation.
🧊 Using Mr. Frosty
A convenient device that allows for controlled freezing rates without electrical power.
1. Prepare cells in cryovials with freeze medium. 2. Place cryovials in Mr. Frosty container. 3. Store at -80°C for 24 hours before transferring to liquid nitrogen.
🧬 Controlled-Rate Freezer
A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.
1. Program the device to gradually decrease the temperature. 2. Place prepared cells in the freezer. 3. After the freezing cycle, transfer cells to liquid nitrogen.
Long-Term Storage
Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.
Ingredients
Contains DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6
Cytion’s Freeze Medium CM-ACF offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.
Key Features of Freeze Medium CM-ACF
Serum-Free Formulation: Eliminates the variability and risks associated with serum, providing a defined and controlled environment for a wide array of cell types, including primary cells, stem cells, and established cell lines.
Broad Compatibility: Effective for a wide array of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-ACF is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Using CM-ACF for Freezing Cells
To use CM-ACF for freezing both adherent and suspension cells, follow these steps:
Preparation:
For adherent cells: Wash and dissociate them from the culture substrate.
For suspension cells: Proceed directly to the next step.
Cell Counting: Ensure cells are at the proper concentration.
Centrifugation: Pellet the cells and resuspend them in CM-ACF freeze medium.
Cryovial Transfer: Transfer the resuspended cells into cryovials.
Freezing Process: Use a slow-freezing method before transferring the cells to long-term storage.
Freezing Methods
Method
Description
Steps
❄️ Manual Freezing
A step-by-step method involving gradual temperature reduction to ensure cell viability.
1. Place cells in freeze medium in a 4°C freezer for 40 minutes. 2. Transfer to a -80°C freezer for 24 hours. 3. Store cells in liquid nitrogen for long-term preservation.
🧊 Using Mr. Frosty
A convenient device that allows for controlled freezing rates without electrical power.
1. Prepare cells in cryovials with freeze medium. 2. Place cryovials in Mr. Frosty container. 3. Store at -80°C for 24 hours before transferring to liquid nitrogen.
🧬 Controlled-Rate Freezer
A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.
1. Program the device to gradually decrease the temperature. 2. Place prepared cells in the freezer. 3. After the freezing cycle, transfer cells to liquid nitrogen.
Long-Term Storage
Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.
Ingredients
Contains DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6
Cytion’s Freeze Medium CM-ACF offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.
Freeze Medium CM-1
From
€59.00*
Cytion’s Freeze Medium CM-1 is a state-of-the-art cryopreservation medium designed to ensure the highest level of cell viability and functionality post-thaw. This versatile medium is suitable for a broad spectrum of cell types, including both human and animal cells, making it an essential tool for diverse research applications. Formulated with a meticulously balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-1 minimizes ice crystal formation and cellular stress during the freezing process, thus preserving cellular integrity.
Key features of Freeze Medium CM-1 include:
Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Using CM-1 for Freezing Cells
To use CM-1 for freezing both adherent and suspension cells, follow these steps:
For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step.
Count the cells to ensure they are at the proper concentration.
Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium.
Transfer the resuspended cells into cryovials.
Use a slow-freezing method before transferring the cells to long-term storage.
🥶 Method
🔍 Description
💡 Steps
❄️
Manual Freezing
A step-by-step method involving gradual temperature reduction to ensure cell viability.
1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes.
2️⃣ Transfer to a -80°C freezer for 24 hours.
3️⃣ Store cells in liquid nitrogen for long-term preservation.
🧊
Using Mr. Frosty
A convenient device that allows for controlled freezing rates without electrical power.
1️⃣ Prepare cells in cryovials with freeze medium.
2️⃣ Place cryovials in Mr. Frosty container.
3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen.
🧬
Controlled-Rate Freezer
A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.
1️⃣ Program the device to gradually decrease the temperature.
2️⃣ Place prepared cells in the freezer.
3️⃣ After the freezing cycle, transfer cells to liquid nitrogen.
Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.
Ingredients
Contains FBS, DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6
Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.
Key features of Freeze Medium CM-1 include:
Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Using CM-1 for Freezing Cells
To use CM-1 for freezing both adherent and suspension cells, follow these steps:
For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step.
Count the cells to ensure they are at the proper concentration.
Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium.
Transfer the resuspended cells into cryovials.
Use a slow-freezing method before transferring the cells to long-term storage.
🥶 Method
🔍 Description
💡 Steps
❄️
Manual Freezing
A step-by-step method involving gradual temperature reduction to ensure cell viability.
1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes.
2️⃣ Transfer to a -80°C freezer for 24 hours.
3️⃣ Store cells in liquid nitrogen for long-term preservation.
🧊
Using Mr. Frosty
A convenient device that allows for controlled freezing rates without electrical power.
1️⃣ Prepare cells in cryovials with freeze medium.
2️⃣ Place cryovials in Mr. Frosty container.
3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen.
🧬
Controlled-Rate Freezer
A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.
1️⃣ Program the device to gradually decrease the temperature.
2️⃣ Place prepared cells in the freezer.
3️⃣ After the freezing cycle, transfer cells to liquid nitrogen.
Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.
Ingredients
Contains FBS, DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6
Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.
Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3
€80.00*
Introducing Ham's F-12K (Kaighn's) Medium, a specialized modification of Ham's F-12 medium designed to meet the unique requirements of biological research. This advanced medium offers distinct advantages, enhancing the cultivation of primary human hepatocytes, as well as rat and chicken liver cells, particularly in reduced serum conditions.
Ham's F-12K (Kaighn's) Medium is carefully formulated to optimize cell culture conditions. It features an enriched composition, providing elevated levels of essential components such as amino acids and sodium pyruvate, as well as additional elements including putrescine, thymidine, hypoxanthine, and zinc. These additions enable researchers to supplement the medium with minimal serum or defined components for specific cell types, facilitating precise experimental conditions.
Notably, Ham's F-12K (Kaighn's) Medium does not contain proteins or growth factors. Consequently, supplementation with growth factors and Fetal Bovine Serum (FBS) is often necessary, allowing researchers to tailor the medium to the requirements of their specific cell lines. For optimal performance, the concentration of FBS must be carefully optimized for each cell line, ensuring optimal growth and functionality.
To maintain physiological pH, Ham's F-12K (Kaighn's) Medium employs a sodium bicarbonate buffer system (2.5 g/L), necessitating a controlled 5-10% CO2 environment during cultivation. This ensures the medium's pH remains within the ideal range for cell growth and viability.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium but the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
135,24
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
105,72
Magnesium sulfate x 7H2O
394,49
Potassium chloride
283,29
Potassium dihydrogen phosphate
58,52
Sodium chloride
7597,20
di-Sodium hydrogen phosphateanhydrous
115,02
Zinc sulfate x 7H2O
0,14
Other Components
D(+)-Glucose anhydrous
1260,00
Hypoxanthine
4,08
DL-α-Lipoic acid
0,21
Phenol red
3,00
Putrescine x 2HCl
0,32
Sodium pyruvate
220,00
NaHCO3
2500,00
Thymidine
0,73
Amino Acids
L-Alanine
17,82
L-Arginine x HCl
421,40
L-Asparagine x H2O
30,02
L-Aspartic acid
26,62
L-Cysteine x HCl x H2O
70,24
L-Glutamine
292,20
L-Glutamic acid
29,42
Glycine
15,01
L-Histidine x HCl x H2O
41,92
L-Isoleucine
7,87
L-Leucine
26,24
L-Lysine x HCl
73,04
L-Methionine
8,95
L-Phenylalanine
9,91
L-Proline
69,06
L-Serine
21,02
L-Threonine
23,82
L-Tryptophan
4,08
L-Tyrosine
10,87
L-Valine
23,42
Vitamins
D(+)-Biotin
0,07
D-Calcium pantothenate
0,48
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
Ham's F-12K (Kaighn's) Medium is carefully formulated to optimize cell culture conditions. It features an enriched composition, providing elevated levels of essential components such as amino acids and sodium pyruvate, as well as additional elements including putrescine, thymidine, hypoxanthine, and zinc. These additions enable researchers to supplement the medium with minimal serum or defined components for specific cell types, facilitating precise experimental conditions.
Notably, Ham's F-12K (Kaighn's) Medium does not contain proteins or growth factors. Consequently, supplementation with growth factors and Fetal Bovine Serum (FBS) is often necessary, allowing researchers to tailor the medium to the requirements of their specific cell lines. For optimal performance, the concentration of FBS must be carefully optimized for each cell line, ensuring optimal growth and functionality.
To maintain physiological pH, Ham's F-12K (Kaighn's) Medium employs a sodium bicarbonate buffer system (2.5 g/L), necessitating a controlled 5-10% CO2 environment during cultivation. This ensures the medium's pH remains within the ideal range for cell growth and viability.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium but the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
135,24
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
105,72
Magnesium sulfate x 7H2O
394,49
Potassium chloride
283,29
Potassium dihydrogen phosphate
58,52
Sodium chloride
7597,20
di-Sodium hydrogen phosphateanhydrous
115,02
Zinc sulfate x 7H2O
0,14
Other Components
D(+)-Glucose anhydrous
1260,00
Hypoxanthine
4,08
DL-α-Lipoic acid
0,21
Phenol red
3,00
Putrescine x 2HCl
0,32
Sodium pyruvate
220,00
NaHCO3
2500,00
Thymidine
0,73
Amino Acids
L-Alanine
17,82
L-Arginine x HCl
421,40
L-Asparagine x H2O
30,02
L-Aspartic acid
26,62
L-Cysteine x HCl x H2O
70,24
L-Glutamine
292,20
L-Glutamic acid
29,42
Glycine
15,01
L-Histidine x HCl x H2O
41,92
L-Isoleucine
7,87
L-Leucine
26,24
L-Lysine x HCl
73,04
L-Methionine
8,95
L-Phenylalanine
9,91
L-Proline
69,06
L-Serine
21,02
L-Threonine
23,82
L-Tryptophan
4,08
L-Tyrosine
10,87
L-Valine
23,42
Vitamins
D(+)-Biotin
0,07
D-Calcium pantothenate
0,48
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
€25.00*
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The unique composition of this RPMI formulation comprises 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and phenol red.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium nitrate x 4H2O
100,00
Magnesium sulfate anhydrous
48,83
Potassium chloride
400,00
Sodium chloride
5,950.00
di-Sodium hydrogen phosphate
800,49
Other Components
D(+)-Glucose anhydrous
2,000.00
Glutathione (red.)
1,00
Phenol red
5,00
NaHCO3
2,000.00
Amino Acids
L-Arginine x HCl
241,86
L-Asparagine x H2O
56,82
L-Aspartic acid
20,00
L-Cystine x 2HCl
65,19
L-Alanyl-L-Glutamine
447,00
L-Glutamic acid
20,00
Glycine
10,00
L-Histidine x HCl x H2O
20,27
L-Hydroxyproline
20,00
L-Isoleucine
50,00
L-Leucine
50,00
L-Lysine x HCl
40,00
L-Methionine
15,00
L-Phenylalanine
15,00
L-Proline
20,00
L-Serine
30,00
L-Threonine
20,00
L-Tryptophan
5,00
L-Tyrosine x 2Na
28,83
L-Valine
20,00
Vitamins
p-Aminobenzoic acid
1,00
D-(+)-Biotin
0,20
D-Calcium pantothenate
0,25
Choline chloride
3,00
Folic acid
1,00
myo-Inositol
35,00
Nicotinamide
1,00
Pyridoxine x HCl
1,00
Riboflavin
0,20
Thiamine x HCl
1,00
Vitamin B12
0.005
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The unique composition of this RPMI formulation comprises 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and phenol red.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium nitrate x 4H2O
100,00
Magnesium sulfate anhydrous
48,83
Potassium chloride
400,00
Sodium chloride
5,950.00
di-Sodium hydrogen phosphate
800,49
Other Components
D(+)-Glucose anhydrous
2,000.00
Glutathione (red.)
1,00
Phenol red
5,00
NaHCO3
2,000.00
Amino Acids
L-Arginine x HCl
241,86
L-Asparagine x H2O
56,82
L-Aspartic acid
20,00
L-Cystine x 2HCl
65,19
L-Alanyl-L-Glutamine
447,00
L-Glutamic acid
20,00
Glycine
10,00
L-Histidine x HCl x H2O
20,27
L-Hydroxyproline
20,00
L-Isoleucine
50,00
L-Leucine
50,00
L-Lysine x HCl
40,00
L-Methionine
15,00
L-Phenylalanine
15,00
L-Proline
20,00
L-Serine
30,00
L-Threonine
20,00
L-Tryptophan
5,00
L-Tyrosine x 2Na
28,83
L-Valine
20,00
Vitamins
p-Aminobenzoic acid
1,00
D-(+)-Biotin
0,20
D-Calcium pantothenate
0,25
Choline chloride
3,00
Folic acid
1,00
myo-Inositol
35,00
Nicotinamide
1,00
Pyridoxine x HCl
1,00
Riboflavin
0,20
Thiamine x HCl
1,00
Vitamin B12
0.005
DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3
€25.00*
DMEM:Ham's F12 is a widely recognized and extensively utilized basal medium in cell culture for biological research. It serves as a fundamental source of nutrients for the growth of various mammalian cell lines, particularly when supplemented with Fetal Bovine Serum (FBS).
This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.
DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.
To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.
DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels. Phenol red enables monitoring of pH changes from 6.2 (yellow) to 8.2 (red).
Quality Control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
154,45
Iron(III)-nitrate x 9H2O
0,05
Iron(II)-sulfate x 7H2O
0,42
Potassium chloride
311,83
Copper(II)-sulfate x 5H2O
0.001
Magnesium chloride anhydrous
28,57
Magnesium sulfate
48,85
Sodium chloride
6,999.50
Sodium dihydrogen phosphate anhydrous
54,35
di-Sodium hydrogen phosphate
70,98
Zinc sulfate x 7H2O
0,43
Other Components
D(+)-Glucose anhydrous
3,151.00
HEPES
3,574.50
Hypoxanthine
2,04
Linoleic acid
0,04
DL-68-Lipoic acid
0.103
Sodium pyruvate
110,00
Phenol red
8,10
Putrescin x 2HCl
0.081
Thymidine
0,36
Amino Acids
L-Alanine
4,45
L-Arginine x HCl
147,35
L-Asparagine x H2O
7,50
L-Aspartic acid
6,65
L-Cystine x 2HCl
31,29
L-Cysteine x HCl x H2O
17,56
L-Glutamine
365,00
L-Glutamic acid
7,35
Glycine
18,75
L-Histidine x HCl x H2O
31,48
L-Isoleucine
54,37
L-Leucine
58,96
L-Lysine x HCl
91,37
L-Methionine
17,24
L-Phenylalanine
35,48
L-Proline
17,27
L-Serine
26,25
L-Threonine
53,55
L-Tryptophan
9,02
L-Tyrosine x 2Na x 2H2O
55,81
L-Valine
52,66
Vitamins
D-(+)-Biotine
0,004
D-Calcium pantothenate
2,12
Choline chloride
8,98
Folic acid
2,66
myo-Inositol
12,51
Nicotinamide
2,02
Pyridoxine x HCl
2,03
Riboflavin
0,22
Thiamine x HCl
2,17
Vitamine B12
0,68
NaHCO3
1,200.00
This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.
DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.
To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.
DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels. Phenol red enables monitoring of pH changes from 6.2 (yellow) to 8.2 (red).
Quality Control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
154,45
Iron(III)-nitrate x 9H2O
0,05
Iron(II)-sulfate x 7H2O
0,42
Potassium chloride
311,83
Copper(II)-sulfate x 5H2O
0.001
Magnesium chloride anhydrous
28,57
Magnesium sulfate
48,85
Sodium chloride
6,999.50
Sodium dihydrogen phosphate anhydrous
54,35
di-Sodium hydrogen phosphate
70,98
Zinc sulfate x 7H2O
0,43
Other Components
D(+)-Glucose anhydrous
3,151.00
HEPES
3,574.50
Hypoxanthine
2,04
Linoleic acid
0,04
DL-68-Lipoic acid
0.103
Sodium pyruvate
110,00
Phenol red
8,10
Putrescin x 2HCl
0.081
Thymidine
0,36
Amino Acids
L-Alanine
4,45
L-Arginine x HCl
147,35
L-Asparagine x H2O
7,50
L-Aspartic acid
6,65
L-Cystine x 2HCl
31,29
L-Cysteine x HCl x H2O
17,56
L-Glutamine
365,00
L-Glutamic acid
7,35
Glycine
18,75
L-Histidine x HCl x H2O
31,48
L-Isoleucine
54,37
L-Leucine
58,96
L-Lysine x HCl
91,37
L-Methionine
17,24
L-Phenylalanine
35,48
L-Proline
17,27
L-Serine
26,25
L-Threonine
53,55
L-Tryptophan
9,02
L-Tyrosine x 2Na x 2H2O
55,81
L-Valine
52,66
Vitamins
D-(+)-Biotine
0,004
D-Calcium pantothenate
2,12
Choline chloride
8,98
Folic acid
2,66
myo-Inositol
12,51
Nicotinamide
2,02
Pyridoxine x HCl
2,03
Riboflavin
0,22
Thiamine x HCl
2,17
Vitamine B12
0,68
NaHCO3
1,200.00
Endothelial Cell Growth Medium
€140.00*
Endothelial cells play a crucial role in the circulatory system by transporting nutrients and waste throughout the body. Researchers and scientists need a medium that can sustain the development and maintenance of endothelial cells in vitro so that they may study their function and malfunction. CLS Endothelial Cell Growth Medium fills this need by creating an environment where endothelial cells may thrive throughout their growth and maintenance phases.
Definition of Endothelial Cell Growth Media
An in-vitro cell culture system requires a particular solution called Endothelial Cell Growth Media to sustain the endothelial cells necessary for the experiment. Endothelial cells in the body are exposed to an environment mimicked by the medium, including necessary and non-essential amino acids, vitamins, hormones, growth factors, and trace minerals. This nutrient-dense fluid is critical for preserving the endothelium phenotype and function because it stimulates cell proliferation. Globally, researchers and scientists rely on the CLS Endothelial Cell Growth Medium because it is both effective and simple.
Importance of Endothelial Cell Research
Blood artery creation, blood pressure control, and wound healing are just a few physiological processes in which endothelial cells play a key part. To keep the blood vessels balanced, these cells may react to chemical and mechanical stimuli. In order to fully comprehend the causes of hypertension, atherosclerosis, and thrombosis, research into endothelial cells is crucial. Investigating endothelial cells may also provide light on how to treat these diseases medically. Providing a stable and uniform environment for endothelial cell development and proliferation, the CLS Endothelial Cell Growth Medium has shown to be an indispensable tool in this kind of study.
Quality Assurance
High-quality in-vitro cell culture media is essential for obtaining consistent and repeatable outcomes. Quality control testing is performed on every CLS Endothelial Cell Growth Media batch to guarantee its uniformity and effectiveness. The sterility, lack of mycoplasma and bacteria, and pH levels of each batch are examined. To promote healthy cell development and proliferation, the medium's pH is kept at 7.2 +/
- 0.02, and the temperature is maintained between 20 and 25 degrees Celsius. To ensure the culture is clear of any contamination that might impact the accuracy of the findings, the medium is also checked for microbiological pollutants such as fungus, bacteria, and mycoplasma.
Maintenance and Disclaimer
The expiration date of CLS Endothelial Cell Growth Media is six weeks from the production date. The medium should be kept at a temperature of between +2°C and +8°C, away from light, and never frozen or heated over 37°C to preserve its quality. As a corollary, it is crucial to avoid microwaves and other unregulated heat sources that might compromise the product's integrity. A portion of the medium may be taken out of the container and brought up to room temperature if just a little amount will be utilized. CLS's Endothelial Cell Growth Medium is a tried and true method for cultivating endothelial cells because of the stringent quality controls used in its production.
Notably, the CLS Endothelial Cell Growth Medium is not for clinical or diagnostic use; it is designed primarily for in-vitro usage. Obtaining accurate and reliable findings from the medium requires strict adherence to the manufacturer's guidelines and quality control methods.
Applications of the Medium
The CLS Endothelial Cell Growth Medium has proven useful in several biological studies. As this medium may be used to cultivate endothelial cells, it can be used to simulate endothelial function and malfunction in organ systems in vitro. This is especially useful for studying the blood-brain barrier or tissue-engineered blood arteries. This is essential for understanding disease processes and creating effective treatments.
Endothelial cells, especially HUVEC cells, have been extensively employed in angiogenesis research, wound healing research, and cancer research, thus, the medium has also been modified to promote their proliferation. Accurate and repeatable findings depend on the consistency and high quality of the medium used to develop these cells, and the CLS Endothelial Cell Growth Media meets both of these requirements.
Definition of Endothelial Cell Growth Media
An in-vitro cell culture system requires a particular solution called Endothelial Cell Growth Media to sustain the endothelial cells necessary for the experiment. Endothelial cells in the body are exposed to an environment mimicked by the medium, including necessary and non-essential amino acids, vitamins, hormones, growth factors, and trace minerals. This nutrient-dense fluid is critical for preserving the endothelium phenotype and function because it stimulates cell proliferation. Globally, researchers and scientists rely on the CLS Endothelial Cell Growth Medium because it is both effective and simple.
Importance of Endothelial Cell Research
Blood artery creation, blood pressure control, and wound healing are just a few physiological processes in which endothelial cells play a key part. To keep the blood vessels balanced, these cells may react to chemical and mechanical stimuli. In order to fully comprehend the causes of hypertension, atherosclerosis, and thrombosis, research into endothelial cells is crucial. Investigating endothelial cells may also provide light on how to treat these diseases medically. Providing a stable and uniform environment for endothelial cell development and proliferation, the CLS Endothelial Cell Growth Medium has shown to be an indispensable tool in this kind of study.
Quality Assurance
High-quality in-vitro cell culture media is essential for obtaining consistent and repeatable outcomes. Quality control testing is performed on every CLS Endothelial Cell Growth Media batch to guarantee its uniformity and effectiveness. The sterility, lack of mycoplasma and bacteria, and pH levels of each batch are examined. To promote healthy cell development and proliferation, the medium's pH is kept at 7.2 +/
- 0.02, and the temperature is maintained between 20 and 25 degrees Celsius. To ensure the culture is clear of any contamination that might impact the accuracy of the findings, the medium is also checked for microbiological pollutants such as fungus, bacteria, and mycoplasma.
Maintenance and Disclaimer
The expiration date of CLS Endothelial Cell Growth Media is six weeks from the production date. The medium should be kept at a temperature of between +2°C and +8°C, away from light, and never frozen or heated over 37°C to preserve its quality. As a corollary, it is crucial to avoid microwaves and other unregulated heat sources that might compromise the product's integrity. A portion of the medium may be taken out of the container and brought up to room temperature if just a little amount will be utilized. CLS's Endothelial Cell Growth Medium is a tried and true method for cultivating endothelial cells because of the stringent quality controls used in its production.
Notably, the CLS Endothelial Cell Growth Medium is not for clinical or diagnostic use; it is designed primarily for in-vitro usage. Obtaining accurate and reliable findings from the medium requires strict adherence to the manufacturer's guidelines and quality control methods.
Applications of the Medium
The CLS Endothelial Cell Growth Medium has proven useful in several biological studies. As this medium may be used to cultivate endothelial cells, it can be used to simulate endothelial function and malfunction in organ systems in vitro. This is especially useful for studying the blood-brain barrier or tissue-engineered blood arteries. This is essential for understanding disease processes and creating effective treatments.
Endothelial cells, especially HUVEC cells, have been extensively employed in angiogenesis research, wound healing research, and cancer research, thus, the medium has also been modified to promote their proliferation. Accurate and repeatable findings depend on the consistency and high quality of the medium used to develop these cells, and the CLS Endothelial Cell Growth Media meets both of these requirements.
McCoys 5A medium (modified), w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3
€30.00*
McCoy's 5A Medium is a highly recommended and specialized medium designed to facilitate the growth and replication of viruses in primary cell cultures. It has gained significant recognition for its exceptional performance in various biological research applications.
One prominent application of McCoy's 5A Medium is its utilization in the culturing of human colon carcinoma cell lines. Specifically, it has been employed in the study of the leucine-rich repeat-containing G-protein-coupled receptor (LGR5) and its role in the metastasis of colon cancer. This medium has been effectively employed in the cultivation of several colon carcinoma cell lines, including HCT116, RKO, FET, CBS, HCT116b, and TENN, enabling researchers to delve deeper into the mechanisms underlying colon cancer metastasis.
In addition to its application in cancer research, McCoy's 5A Medium has proven to be indispensable in the study of osteoblasts. Researchers investigating the ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media have utilized this medium to culture osteoblasts. This application has facilitated a better understanding of the interactions between osteoblasts and calcium-deficient hydroxyapatite, contributing to advancements in the field of bone research.
Notably, McCoy's 5A Medium was meticulously formulated by modifying the amino acids found in Basal Medium Eagle to provide optimal support for liver tumor cells. This enriched formulation enables its suitability for a diverse range of established cell lines, as well as primary cells, further enhancing its versatility and applicability in various research settings.
Moreover, McCoy's 5A Medium extends its biochemical and physiological effects beyond liver tumor cells. It has been successfully employed to support growth in primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenal, lung, spleen, rat embryo, and other cell types. This wide range of applications attests to the broad utility of McCoy's 5A Medium in supporting the growth and maintenance of various cell types for comprehensive biological research.
Formulation
This McCoy's 5A medium (modified) contains 3.0 g/L of Glucose, stable Glutamine, 2.0 mM of Sodium pyruvate, and 2.2 g/L of NaHCO3.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
132,46
Magnesium sulfate x 7H2O
200,00
Potassium chloride
400,00
Sodium chloride
6,460.00
Sodium dihydrogen phosphate x H2O
580,00
Other Components
D(+)-Galactose anhydrous
3,000.00
Glutathione (red.)
0,50
Bacto-Peptone
600,00
Phenol red
10,00
Amino Acids
L-Alanine
13,36
L-Arginine x HCl
42,10
L-Asparagine x H2O
45,00
L-Aspartic acid
19,97
L-Cysteine
24,24
L-Glutamine stable
326,61
L-Glutamic acid
22,10
Glycine
7,50
L-Histidine x HCl x H2O
20,76
L-Hydroxyproline
19,70
L-Isoleucine
39,36
L-Leucine
39,36
L-Lysine x HCl
36,50
L-Methionine
14,90
L-Phenylalanine
16,50
L-Proline
17,30
L-Serine
26,30
L-Threonine
17,90
L-Tryptophan
3,10
L-Tyrosine
18,10
L-Valine
17,60
Vitamins
p-Aminobenzoic acid
1,00
Ascorbic acid
0,50
D(+)-Biotin
0,20
D-Calcium pantothenate
0,20
Choline chloride
5,00
One prominent application of McCoy's 5A Medium is its utilization in the culturing of human colon carcinoma cell lines. Specifically, it has been employed in the study of the leucine-rich repeat-containing G-protein-coupled receptor (LGR5) and its role in the metastasis of colon cancer. This medium has been effectively employed in the cultivation of several colon carcinoma cell lines, including HCT116, RKO, FET, CBS, HCT116b, and TENN, enabling researchers to delve deeper into the mechanisms underlying colon cancer metastasis.
In addition to its application in cancer research, McCoy's 5A Medium has proven to be indispensable in the study of osteoblasts. Researchers investigating the ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media have utilized this medium to culture osteoblasts. This application has facilitated a better understanding of the interactions between osteoblasts and calcium-deficient hydroxyapatite, contributing to advancements in the field of bone research.
Notably, McCoy's 5A Medium was meticulously formulated by modifying the amino acids found in Basal Medium Eagle to provide optimal support for liver tumor cells. This enriched formulation enables its suitability for a diverse range of established cell lines, as well as primary cells, further enhancing its versatility and applicability in various research settings.
Moreover, McCoy's 5A Medium extends its biochemical and physiological effects beyond liver tumor cells. It has been successfully employed to support growth in primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenal, lung, spleen, rat embryo, and other cell types. This wide range of applications attests to the broad utility of McCoy's 5A Medium in supporting the growth and maintenance of various cell types for comprehensive biological research.
Formulation
This McCoy's 5A medium (modified) contains 3.0 g/L of Glucose, stable Glutamine, 2.0 mM of Sodium pyruvate, and 2.2 g/L of NaHCO3.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
132,46
Magnesium sulfate x 7H2O
200,00
Potassium chloride
400,00
Sodium chloride
6,460.00
Sodium dihydrogen phosphate x H2O
580,00
Other Components
D(+)-Galactose anhydrous
3,000.00
Glutathione (red.)
0,50
Bacto-Peptone
600,00
Phenol red
10,00
Amino Acids
L-Alanine
13,36
L-Arginine x HCl
42,10
L-Asparagine x H2O
45,00
L-Aspartic acid
19,97
L-Cysteine
24,24
L-Glutamine stable
326,61
L-Glutamic acid
22,10
Glycine
7,50
L-Histidine x HCl x H2O
20,76
L-Hydroxyproline
19,70
L-Isoleucine
39,36
L-Leucine
39,36
L-Lysine x HCl
36,50
L-Methionine
14,90
L-Phenylalanine
16,50
L-Proline
17,30
L-Serine
26,30
L-Threonine
17,90
L-Tryptophan
3,10
L-Tyrosine
18,10
L-Valine
17,60
Vitamins
p-Aminobenzoic acid
1,00
Ascorbic acid
0,50
D(+)-Biotin
0,20
D-Calcium pantothenate
0,20
Choline chloride
5,00
Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS
€40.00*
Medium 199 is a versatile and widely used cell culture medium specially formulated for the cultivation of primary explants. This comprehensive medium combines essential vitamins, amino acids, and other factors to provide a fully defined nutrient source for a variety of cell types. It is particularly suitable for non-transformed cells, making it an invaluable tool for biological research.
Medium 199 offers a range of applications in the field. It can effectively maintain the cumulus-oocyte complex (COC) and support the in vitro maturation of oocytes. Additionally, it is employed in the rinsing of aspiration lines during ovum collection from German Holstein cows. Moreover, Medium 199 serves as an excellent medium for the culture of cardiac endothelial cells derived from rats. These applications demonstrate the versatility and adaptability of Medium 199 to various experimental needs.
History
The development of Medium 199 in the 1950s marked a significant advancement in tissue culture media. Prior to its introduction, many culture media relied on animal-derived products and tissue extracts. However, Morgan and colleagues revolutionized the field by formulating a completely defined nutritional source for cell cultures. Through their experiments involving different combinations of vitamins, amino acids, and other factors, they discovered the exceptional growth-promoting properties of Medium 199.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
264,92
Iron (III) nitrate x 9H2O
0,72
Magnesium sulfate
97,67
Potassium chloride
400,00
Sodium acetat x 3H2O
82,95
Sodium chloride
6,800.00
Sodium dihydrogen phosphate x H2O
140,00
Other Components
Adenine sulfate
10,00
AMP
0,20
ATP
1,00
Cholesterol
0,20
2‘-Deoxyribose
0,50
D(+)-Glucose anhydrous
1,000.00
Glutathione (red.)
0,05
Guanine x HCl
0,30
Hypoxanthine
0,30
Phenol red
10,00
D-Ribose
0,50
Thymine
0,30
Tween 80
4,90
Uracil
0,30
Xanthine
0,30
NaHCO3
2,200.00
Amino Acids
L-Alanine
25,00
L-Arginine x HCl
70,00
L-Aspartic acid
30,00
L-Cysteine x HCl x H2O
0,10
L-Cystine
20,00
L-Glutamine stable
149,00
L-Glutamic acid
67,00
Glycine
50,00
L-Histidine x HCl x H2O
21,88
L-Hydroxyproline
10,00
L-Isoleucine
20,00
L-Leucine
60,00
L-Lysine x HCl
70,00
L-Methionine
15,00
L-Phenylalanine
25,00
L-Proline
40,00
L-Serine
25,00
L-Threonine
30,00
L-Tryptophan
10,00
L-Tyrosine
40,00
L-Valine
25,00
Vitamins
4-Amino benzoic acid
0,05
Ascorbic acid
0,05
D(+)-Biotin
0,01
Calciferol
0,10
D-Calcium pantothenate
0,01
Choline chloride
0,50
Folic acid
0,01
myo-Inositol
0,05
Menadione
0,01
Nicotinic acid
0.025
Nicotinamide
0.025
Pyridoxal x HCl
0.025
Pyridoxol x HCl
0.025
Riboflavin
0,01
DL-α-Tocopherol phosphate disodium salt
0,01
Thiamine x HCl
0,01
Vitamine A acetate
0,14
Medium 199 offers a range of applications in the field. It can effectively maintain the cumulus-oocyte complex (COC) and support the in vitro maturation of oocytes. Additionally, it is employed in the rinsing of aspiration lines during ovum collection from German Holstein cows. Moreover, Medium 199 serves as an excellent medium for the culture of cardiac endothelial cells derived from rats. These applications demonstrate the versatility and adaptability of Medium 199 to various experimental needs.
History
The development of Medium 199 in the 1950s marked a significant advancement in tissue culture media. Prior to its introduction, many culture media relied on animal-derived products and tissue extracts. However, Morgan and colleagues revolutionized the field by formulating a completely defined nutritional source for cell cultures. Through their experiments involving different combinations of vitamins, amino acids, and other factors, they discovered the exceptional growth-promoting properties of Medium 199.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
264,92
Iron (III) nitrate x 9H2O
0,72
Magnesium sulfate
97,67
Potassium chloride
400,00
Sodium acetat x 3H2O
82,95
Sodium chloride
6,800.00
Sodium dihydrogen phosphate x H2O
140,00
Other Components
Adenine sulfate
10,00
AMP
0,20
ATP
1,00
Cholesterol
0,20
2‘-Deoxyribose
0,50
D(+)-Glucose anhydrous
1,000.00
Glutathione (red.)
0,05
Guanine x HCl
0,30
Hypoxanthine
0,30
Phenol red
10,00
D-Ribose
0,50
Thymine
0,30
Tween 80
4,90
Uracil
0,30
Xanthine
0,30
NaHCO3
2,200.00
Amino Acids
L-Alanine
25,00
L-Arginine x HCl
70,00
L-Aspartic acid
30,00
L-Cysteine x HCl x H2O
0,10
L-Cystine
20,00
L-Glutamine stable
149,00
L-Glutamic acid
67,00
Glycine
50,00
L-Histidine x HCl x H2O
21,88
L-Hydroxyproline
10,00
L-Isoleucine
20,00
L-Leucine
60,00
L-Lysine x HCl
70,00
L-Methionine
15,00
L-Phenylalanine
25,00
L-Proline
40,00
L-Serine
25,00
L-Threonine
30,00
L-Tryptophan
10,00
L-Tyrosine
40,00
L-Valine
25,00
Vitamins
4-Amino benzoic acid
0,05
Ascorbic acid
0,05
D(+)-Biotin
0,01
Calciferol
0,10
D-Calcium pantothenate
0,01
Choline chloride
0,50
Folic acid
0,01
myo-Inositol
0,05
Menadione
0,01
Nicotinic acid
0.025
Nicotinamide
0.025
Pyridoxal x HCl
0.025
Pyridoxol x HCl
0.025
Riboflavin
0,01
DL-α-Tocopherol phosphate disodium salt
0,01
Thiamine x HCl
0,01
Vitamine A acetate
0,14
IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3
€25.00*
Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH.
IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.
IMDM vs. DMEM
IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.
IMDM vs. RPMI
IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2 H2O
219,00
Potassium chloride
330,00
Potassium nitrate
0.076
Magnesium sulfate anhydrous
97,73
Sodium chloride
4,505.00
Sodium dihydrogen phosphate anhydrous
109,00
Sodium selenite
0,02
Other Components
D(+)-Glucose anhydrous
4,500.00
HEPES
5,958.00
Sodium pyruvate
110,00
Phenol red
15,00
Amino Acids
L-Alanine
25,00
L-Arginine x HCl
84,00
L-Asparagine x H2O
25,00
L-Aspartic acid
30,00
L-Cystine x 2HCl
91,24
L-Glutamine
584,00
L-Glutamic acid
75,00
Glycine
30,00
L-Histidine x HCl x H2O
42,00
L-Isoleucine
104,80
L-Leucine
104,80
L-Lysine x HCl
146,20
L-Methionine
30,00
L-Phenylalanine
66,00
L-Proline
40,00
L-Serine
42,00
L-Threonine
95,20
L-Tryptophan
16,00
L-Tyrosine x 2Na
104,20
L-Valine
93,60
Vitamins
D(+)-Biotin
0.013
D-Calcium pantothenate
4,00
Choline chloride
4,00
Folic acid
4,00
myo-Inositol
7,20
IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.
IMDM vs. DMEM
IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.
IMDM vs. RPMI
IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2 H2O
219,00
Potassium chloride
330,00
Potassium nitrate
0.076
Magnesium sulfate anhydrous
97,73
Sodium chloride
4,505.00
Sodium dihydrogen phosphate anhydrous
109,00
Sodium selenite
0,02
Other Components
D(+)-Glucose anhydrous
4,500.00
HEPES
5,958.00
Sodium pyruvate
110,00
Phenol red
15,00
Amino Acids
L-Alanine
25,00
L-Arginine x HCl
84,00
L-Asparagine x H2O
25,00
L-Aspartic acid
30,00
L-Cystine x 2HCl
91,24
L-Glutamine
584,00
L-Glutamic acid
75,00
Glycine
30,00
L-Histidine x HCl x H2O
42,00
L-Isoleucine
104,80
L-Leucine
104,80
L-Lysine x HCl
146,20
L-Methionine
30,00
L-Phenylalanine
66,00
L-Proline
40,00
L-Serine
42,00
L-Threonine
95,20
L-Tryptophan
16,00
L-Tyrosine x 2Na
104,20
L-Valine
93,60
Vitamins
D(+)-Biotin
0.013
D-Calcium pantothenate
4,00
Choline chloride
4,00
Folic acid
4,00
myo-Inositol
7,20
Ham's F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
€25.00*
Ham's F-12 Medium, also known as Ham's F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA).
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
44,00
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
122,00
Potassium chloride
223,65
Sodium chloride
7599,00
di-Sodium hydrogen phosphateanhydrous
142,04
Zinc sulfate x 7H2O
0,86
Other Components
D(+)-Glucose anhydrous
1801,60
Hypoxanthine
4,08
Linoleic acid
0,08
DL-α-Lipoic acid
0,21
Phenol red
1,20
Putrescine x 2HCl
0,16
Sodium pyruvate
110,00
Thymidine
0,73
NaHCO3
1176,00
Amino Acids
L-Alanine
8,91
L-Arginine x HCl
210,70
L-Asparagine x H2O
15,01
L-Aspartic acid
13,31
L-Cysteine x HCl x H2O
35,12
L-Alanyl-L-Glutamine
217,30
L-Glutamic acid
14,71
Glycine
7,51
L-Histidine x HCl x H2O
20,96
L-Isoleucine
3,94
L-Leucine
13,12
L-Lysine x HCl
36,54
L-Methionine
4,48
L-Phenylalanine
4,96
L-Proline
34,53
L-Serine
10,51
L-Threonine
11,91
L-Tryptophan
2,04
L-Tyrosine
5,44
L-Valine
11,71
Vitamins
D(+)-Biotin
0,01
D-Calcium pantothenate
0,24
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
44,00
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
122,00
Potassium chloride
223,65
Sodium chloride
7599,00
di-Sodium hydrogen phosphateanhydrous
142,04
Zinc sulfate x 7H2O
0,86
Other Components
D(+)-Glucose anhydrous
1801,60
Hypoxanthine
4,08
Linoleic acid
0,08
DL-α-Lipoic acid
0,21
Phenol red
1,20
Putrescine x 2HCl
0,16
Sodium pyruvate
110,00
Thymidine
0,73
NaHCO3
1176,00
Amino Acids
L-Alanine
8,91
L-Arginine x HCl
210,70
L-Asparagine x H2O
15,01
L-Aspartic acid
13,31
L-Cysteine x HCl x H2O
35,12
L-Alanyl-L-Glutamine
217,30
L-Glutamic acid
14,71
Glycine
7,51
L-Histidine x HCl x H2O
20,96
L-Isoleucine
3,94
L-Leucine
13,12
L-Lysine x HCl
36,54
L-Methionine
4,48
L-Phenylalanine
4,96
L-Proline
34,53
L-Serine
10,51
L-Threonine
11,91
L-Tryptophan
2,04
L-Tyrosine
5,44
L-Valine
11,71
Vitamins
D(+)-Biotin
0,01
D-Calcium pantothenate
0,24
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
NCI-H295R Cell Growth Medium
€150.00*
NCI-H295R Cell Growth Medium
Discover the advanced NCI-H295R Cell Growth Medium, an expertly crafted nutrient solution specifically designed to foster the growth and maintenance of mammalian cell lines, including the specialized NCI-H295R cells. This medium stands out with its unique blend of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Nutrient Mixture F-12) in a precise 1:1 ratio, enriched with essential supplements to meet the intricate needs of cell culture.
Enhanced Formulation for Superior Cell Culture Performance
Supplemented Baseline: The basic medium is already rich, combining DMEM's high amino acid and vitamin concentrations with the diverse nutrients of Ham's F-12. To this potent mix, we've added 5% Fetal Bovine Serum (FBS), providing crucial growth factors, hormones, and proteins.
Tailored Additives: Beyond FBS, this medium is fortified with critical supplements including Insulin, Transferrin, Bovine Serum Albumin, Selenious Acid, and Lineloic Acid. These additions cater to specific cellular requirements, promoting enhanced cell growth, metabolic function, and overall health.
L-Glutamine Boost: The presence of L-glutamine further amplifies the medium's efficacy, supporting essential metabolic processes and encouraging robust cell proliferation.
Optimized for Cell Culture
pH Controlled Environment: Incorporating a bicarbonate buffer system and phenol red indicator, the medium ensures a stable pH environment. It is optimized for a 5% CO2 atmosphere, allowing for precise pH monitoring and adjustment.
Quality and Purity: Each lot of basic medium undergoes rigorous testing for sterility, mycoplasma, and bacteria, ensuring the medium's integrity. With a pH maintained medium, you can trust in the consistent quality and performance of this medium.
Handling and Storage
Maintain the medium's quality by storing it refrigerated at +2°C to +8°C, away from light. Avoid extremes, such as freezing or overheating, to preserve its efficacy. For partial use, gently warm the needed quantity at room temperature, ensuring the medium is brought to the appropriate condition without compromising its components.
Shelf life
With an 8-week shelf life from the manufacturing date, the NCI-H295R Cell Growth Medium is a reliable choice for your cell culture needs, ensuring the vitality and productivity of your research.
Discover the advanced NCI-H295R Cell Growth Medium, an expertly crafted nutrient solution specifically designed to foster the growth and maintenance of mammalian cell lines, including the specialized NCI-H295R cells. This medium stands out with its unique blend of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Nutrient Mixture F-12) in a precise 1:1 ratio, enriched with essential supplements to meet the intricate needs of cell culture.
Enhanced Formulation for Superior Cell Culture Performance
Supplemented Baseline: The basic medium is already rich, combining DMEM's high amino acid and vitamin concentrations with the diverse nutrients of Ham's F-12. To this potent mix, we've added 5% Fetal Bovine Serum (FBS), providing crucial growth factors, hormones, and proteins.
Tailored Additives: Beyond FBS, this medium is fortified with critical supplements including Insulin, Transferrin, Bovine Serum Albumin, Selenious Acid, and Lineloic Acid. These additions cater to specific cellular requirements, promoting enhanced cell growth, metabolic function, and overall health.
L-Glutamine Boost: The presence of L-glutamine further amplifies the medium's efficacy, supporting essential metabolic processes and encouraging robust cell proliferation.
Optimized for Cell Culture
pH Controlled Environment: Incorporating a bicarbonate buffer system and phenol red indicator, the medium ensures a stable pH environment. It is optimized for a 5% CO2 atmosphere, allowing for precise pH monitoring and adjustment.
Quality and Purity: Each lot of basic medium undergoes rigorous testing for sterility, mycoplasma, and bacteria, ensuring the medium's integrity. With a pH maintained medium, you can trust in the consistent quality and performance of this medium.
Handling and Storage
Maintain the medium's quality by storing it refrigerated at +2°C to +8°C, away from light. Avoid extremes, such as freezing or overheating, to preserve its efficacy. For partial use, gently warm the needed quantity at room temperature, ensuring the medium is brought to the appropriate condition without compromising its components.
Shelf life
With an 8-week shelf life from the manufacturing date, the NCI-H295R Cell Growth Medium is a reliable choice for your cell culture needs, ensuring the vitality and productivity of your research.
DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
€25.00*
DMEM (Dulbecco's Modified Eagle Medium) is a highly versatile and widely utilized basal medium designed to support the growth of a diverse range of mammalian cells in biological research. It serves as an ideal medium for culturing primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, as well as popular cell lines like HeLa, 293, Cos-7, and PC-12.
What sets DMEM apart from other media is its unique composition. It contains an impressive fourfold increase in amino acid and vitamin concentration compared to the original Eagle's Minimal Essential Medium. Initially developed with low glucose (1 g/L) and sodium pyruvate, DMEM is frequently employed with higher glucose levels, either with or without sodium pyruvate. Notably, DMEM does not contain proteins, lipids, or growth factors, necessitating supplementation. To achieve optimal growth, a common approach is to supplement DMEM with 10% Fetal Bovine Serum (FBS). Additionally, DMEM employs a sodium bicarbonate buffer system (3.7 g/L), requiring a 5-10% CO2 environment to maintain a physiological pH.
Dulbecco's Modified Eagle Medium (DMEM) is highly regarded among defined media for cell and tissue culture, catering to the growth needs of various adherent cell phenotypes. It surpasses the original Eagle's Medium, developed in the 1950s for cultivating chicken cells, through the enhanced supplementary formulation known as Dulbecco's modification. This modification significantly elevates the content of select amino acids and vitamins up to fourfold compared to the original medium.
In the field of cell culture, DMEM plays a vital role as a growth medium for different cell types, including primary cells, stem cells, and transformed cells. Researchers also employ the modified version of DMEM for a wide array of research applications, such as drug discovery, tissue engineering, and the study of cell signaling pathways.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride anhydrous
200,00
Iron (III) nitrate x 9H2O
0,10
Magnesium sulfate anhydrous
97,66
Potassium chloride
400,00
Sodium chloride
6.400,00
Sodium dihydrogen phosphateanhydrous
108,69
Other Components
D(+)-Glucose anhydrous
4.500,00
Sodium pyruvate
110,00
Phenol red
15,00
NaHCO3
1.500,00
Amino acids
L-Arginine x HCl
84,00
L-Cystine x 2HCl
62,58
L-Glutamine
584,00
Glycine
30,00
L-Histidine x HCl x H2O
42,00
L-Isoleucine
104,80
L-Leucine
104,80
L-Lysine x HCl
146,20
L-Methionine
30,00
L-Phenylalanine
66,00
L-Serine
42,00
L-Threonine
95,20
L-Tryptophan
16,00
L-Tyrosine x Na
103,79
L-Valine
93,60
Vitamins
D-Calcium pantothenate
4,00
Choline chloride
4,00
Folic acid
4,00
myo-Inositol
7,00
Nicotinamide
4,00
Pyridoxine x HCl
4,00
Riboflavin
0,40
Thiamine x HCl
4,00
What sets DMEM apart from other media is its unique composition. It contains an impressive fourfold increase in amino acid and vitamin concentration compared to the original Eagle's Minimal Essential Medium. Initially developed with low glucose (1 g/L) and sodium pyruvate, DMEM is frequently employed with higher glucose levels, either with or without sodium pyruvate. Notably, DMEM does not contain proteins, lipids, or growth factors, necessitating supplementation. To achieve optimal growth, a common approach is to supplement DMEM with 10% Fetal Bovine Serum (FBS). Additionally, DMEM employs a sodium bicarbonate buffer system (3.7 g/L), requiring a 5-10% CO2 environment to maintain a physiological pH.
Dulbecco's Modified Eagle Medium (DMEM) is highly regarded among defined media for cell and tissue culture, catering to the growth needs of various adherent cell phenotypes. It surpasses the original Eagle's Medium, developed in the 1950s for cultivating chicken cells, through the enhanced supplementary formulation known as Dulbecco's modification. This modification significantly elevates the content of select amino acids and vitamins up to fourfold compared to the original medium.
In the field of cell culture, DMEM plays a vital role as a growth medium for different cell types, including primary cells, stem cells, and transformed cells. Researchers also employ the modified version of DMEM for a wide array of research applications, such as drug discovery, tissue engineering, and the study of cell signaling pathways.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride anhydrous
200,00
Iron (III) nitrate x 9H2O
0,10
Magnesium sulfate anhydrous
97,66
Potassium chloride
400,00
Sodium chloride
6.400,00
Sodium dihydrogen phosphateanhydrous
108,69
Other Components
D(+)-Glucose anhydrous
4.500,00
Sodium pyruvate
110,00
Phenol red
15,00
NaHCO3
1.500,00
Amino acids
L-Arginine x HCl
84,00
L-Cystine x 2HCl
62,58
L-Glutamine
584,00
Glycine
30,00
L-Histidine x HCl x H2O
42,00
L-Isoleucine
104,80
L-Leucine
104,80
L-Lysine x HCl
146,20
L-Methionine
30,00
L-Phenylalanine
66,00
L-Serine
42,00
L-Threonine
95,20
L-Tryptophan
16,00
L-Tyrosine x Na
103,79
L-Valine
93,60
Vitamins
D-Calcium pantothenate
4,00
Choline chloride
4,00
Folic acid
4,00
myo-Inositol
7,00
Nicotinamide
4,00
Pyridoxine x HCl
4,00
Riboflavin
0,40
Thiamine x HCl
4,00