HEK293 suspension-adapted
























General information
Description | The HEK293 suspension-adapted cell line is a variant of the human embryonic kidney 293 (HEK293) cells that has been modified to grow in suspension culture rather than adherent culture. This adaptation is significant for industrial applications where large-scale protein production is required. The cells maintain many of the characteristics of the original HEK293 line, including a robust transient transfection efficiency and the ability to post-translationally modify expressed proteins in a manner similar to that of native human cells. These cells are particularly valued in the biotechnology and pharmaceutical industries for the production of recombinant proteins and viruses for gene therapy and vaccine development. The adaptation to suspension culture allows for easier scalability and simplifies the harvesting process, making it more suitable for commercial-scale bioprocessing. The HEK293 suspension-adapted cell line supports various viral production systems, including adenovirus, lentivirus, and adeno-associated virus (AAV), which are pivotal in therapeutic applications and research. Overall, the HEK293 suspension-adapted cell line is a crucial tool in the fields of molecular biology and bioprocessing, providing a versatile platform for the production of various biologically active molecules. Its ease of genetic manipulation and ability to produce proteins that are correctly folded and post-translationally modified according to human cell patterns make it an indispensable resource in many advanced therapeutic and research settings. |
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Organism | Human |
Tissue | Kidney |
Applications | Transfection host |
Characteristics
Age | Fetus |
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Gender | Female |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | HEK293 suspension-adapted (Cytion catalog number 300686) |
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Biosafety level | 1 |
NCBI_TaxID | 9606 |
CellosaurusAccession | CVCL_0045 |
Expression / Mutation
Receptors expressed | Vitronectin |
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Protein expression | CEA negative, p53 positive |
Tumorigenic | In nude mice |
Virus susceptibility | transformed with adenovirus 5 DNA adenovirus 5 DNA |
Handling
Culture Medium | Panserin 293S (from PanBiotech) |
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Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 3 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
D7S820: 11,12
TH01: 7,9.3
TPOX: 11
vWA: 16,19
D3S1358: 15,17
D21S11: 28,30.2
D18S51: 18
Penta E: 7,15
Penta D: 9,1
D8S1179: 12,14
FGA: 23
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