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IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3


Content: 500 milliliter
Product number: 820800a
Product information "IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3"

Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH.

IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.


IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.


IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.

Quality control

  • pH = 7.2 +/- 0.02 at 20-25°C. 
  • Each lot has been tested for sterility and absence of mycoplasma and bacteria.


  • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
  • Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
  • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
  • Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.


Components mg/L
Inorganic Salts Calcium chloride x 2 H2O 219,00
Potassium chloride 330,00
Potassium nitrate 0.076
Magnesium sulfate anhydrous 97,73
Sodium chloride 4,505.00
Sodium dihydrogen phosphate anhydrous 109,00
Sodium selenite 0,02
Other Components D(+)-Glucose anhydrous 4,500.00
HEPES 5,958.00
Sodium pyruvate 110,00
Phenol red 15,00
Amino Acids L-Alanine 25,00
L-Arginine x HCl 84,00
L-Asparagine x H2O 25,00
L-Aspartic acid 30,00
L-Cystine x 2HCl 91,24
L-Glutamine 584,00
L-Glutamic acid 75,00
Glycine 30,00
L-Histidine x HCl x H2O 42,00
L-Isoleucine 104,80
L-Leucine 104,80
L-Lysine x HCl 146,20
L-Methionine 30,00
L-Phenylalanine 66,00
L-Proline 40,00
L-Serine 42,00
L-Threonine 95,20
L-Tryptophan 16,00
L-Tyrosine x 2Na 104,20
L-Valine 93,60
Vitamins D(+)-Biotin 0.013
D-Calcium pantothenate 4,00
Choline chloride 4,00
Folic acid 4,00
myo-Inositol 7,20

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