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RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3


Content: 500 milliliter
Product number: 820700a
Product information "RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3"

RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).

Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.

The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.

The unique composition of this RPMI formulation comprises 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and phenol red.

Quality control

  • pH = 7.2 +/- 0.02 at 20-25°C. 
  • Each lot has been tested for sterility and absence of mycoplasma and bacteria.


  • Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
  • Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
  • If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
  • Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.


Components mg/L
Inorganic Salts Calcium nitrate x 4H2O 100,00
Magnesium sulfate anhydrous 48,83
Potassium chloride 400,00
Sodium chloride 5,950.00
di-Sodium hydrogen phosphate 800,49
Other Components D(+)-Glucose anhydrous 2,000.00
Glutathione (red.) 1,00
Phenol red 5,00
NaHCO3 2,000.00
Amino Acids L-Arginine x HCl 241,86
L-Asparagine x H2O 56,82
L-Aspartic acid 20,00
L-Cystine x 2HCl 65,19
L-Alanyl-L-Glutamine 447,00
L-Glutamic acid 20,00
Glycine 10,00
L-Histidine x HCl x H2O 20,27
L-Hydroxyproline 20,00
L-Isoleucine 50,00
L-Leucine 50,00
L-Lysine x HCl 40,00
L-Methionine 15,00
L-Phenylalanine 15,00
L-Proline 20,00
L-Serine 30,00
L-Threonine 20,00
L-Tryptophan 5,00
L-Tyrosine x 2Na 28,83
L-Valine 20,00
Vitamins p-Aminobenzoic acid 1,00
D-(+)-Biotin 0,20
D-Calcium pantothenate 0,25
Choline chloride 3,00
Folic acid 1,00
myo-Inositol 35,00
Nicotinamide 1,00
Pyridoxine x HCl 1,00
Riboflavin 0,20
Thiamine x HCl 1,00
Vitamin B12 0.005

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