Freeze medium CM-ACF - serum free - 500 ml
Cytion’s Freeze Medium CM-ACF is an advanced serum-free cryopreservation medium tailored to ensure exceptional cell viability and functionality post-thaw. This versatile medium is suitable for a wide range of cell types, including both human and animal cells, making it an indispensable tool for diverse research applications. Formulated with a carefully balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-ACF minimizes ice crystal formation and cellular stress during the freezing process, thereby preserving cellular integrity.
Key Features of Freeze Medium CM-ACF
- Serum-Free Formulation: Eliminates the variability and risks associated with serum, providing a defined and controlled environment for a wide array of cell types, including primary cells, stem cells, and established cell lines.
- Broad Compatibility: Effective for a wide array of cell types, including primary cells, stem cells, and established cell lines.
- High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
- Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
- Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
- Long Shelf Life: CM-ACF is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Using CM-ACF for Freezing Cells
To use CM-ACF for freezing both adherent and suspension cells, follow these steps:
- Preparation:
- For adherent cells: Wash and dissociate them from the culture substrate.
- For suspension cells: Proceed directly to the next step.
- Cell Counting: Ensure cells are at the proper concentration.
- Centrifugation: Pellet the cells and resuspend them in CM-ACF freeze medium.
- Cryovial Transfer: Transfer the resuspended cells into cryovials.
- Freezing Process: Use a slow-freezing method before transferring the cells to long-term storage.
Freezing Methods
| Method | Description | Steps |
|---|---|---|
| ❄️ Manual Freezing | A step-by-step method involving gradual temperature reduction to ensure cell viability. | 1. Place cells in freeze medium in a 4°C freezer for 40 minutes. 2. Transfer to a -80°C freezer for 24 hours. 3. Store cells in liquid nitrogen for long-term preservation. |
| 🧊 Using Mr. Frosty | A convenient device that allows for controlled freezing rates without electrical power. | 1. Prepare cells in cryovials with freeze medium. 2. Place cryovials in Mr. Frosty container. 3. Store at -80°C for 24 hours before transferring to liquid nitrogen. |
| 🧬 Controlled-Rate Freezer | A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction. | 1. Program the device to gradually decrease the temperature. 2. Place prepared cells in the freezer. 3. After the freezing cycle, transfer cells to liquid nitrogen. |
Long-Term Storage
Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.
Ingredients
- Contains DMSO, Glucose, Salts
- Buffering capacity: pH = 7.2 to 7.6
Cytion’s Freeze Medium CM-ACF offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.