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Freeze medium CM-ACF - serum free - 100 ml


Content: 1 100ml
Product nr Options Price
806050 50 ml €59.00
806100 100 ml €99.00
806500 500 ml €450.00
Product number: 806100
Product information "Freeze medium CM-ACF - serum free - 100 ml"

Cytion’s Freeze Medium CM-ACF is an advanced serum-free cryopreservation medium tailored to ensure exceptional cell viability and functionality post-thaw. This versatile medium is suitable for a wide range of cell types, including both human and animal cells, making it an indispensable tool for diverse research applications. Formulated with a carefully balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-ACF minimizes ice crystal formation and cellular stress during the freezing process, thereby preserving cellular integrity.

Key Features of Freeze Medium CM-ACF

  • Serum-Free Formulation: Eliminates the variability and risks associated with serum, providing a defined and controlled environment for a wide array of cell types, including primary cells, stem cells, and established cell lines.
  • Broad Compatibility: Effective for a wide array of cell types, including primary cells, stem cells, and established cell lines.
  • High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
  • Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
  • Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
  • Long Shelf Life: CM-ACF is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.

Using CM-ACF for Freezing Cells

To use CM-ACF for freezing both adherent and suspension cells, follow these steps:

  1. Preparation:
    • For adherent cells: Wash and dissociate them from the culture substrate.
    • For suspension cells: Proceed directly to the next step.
  2. Cell Counting: Ensure cells are at the proper concentration.
  3. Centrifugation: Pellet the cells and resuspend them in CM-ACF freeze medium.
  4. Cryovial Transfer: Transfer the resuspended cells into cryovials.
  5. Freezing Process: Use a slow-freezing method before transferring the cells to long-term storage.

Freezing Methods

Method Description Steps
❄️ Manual Freezing A step-by-step method involving gradual temperature reduction to ensure cell viability. 1. Place cells in freeze medium in a 4°C freezer for 40 minutes. 2. Transfer to a -80°C freezer for 24 hours. 3. Store cells in liquid nitrogen for long-term preservation.
🧊 Using Mr. Frosty A convenient device that allows for controlled freezing rates without electrical power. 1. Prepare cells in cryovials with freeze medium. 2. Place cryovials in Mr. Frosty container. 3. Store at -80°C for 24 hours before transferring to liquid nitrogen.
🧬 Controlled-Rate Freezer A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction. 1. Program the device to gradually decrease the temperature. 2. Place prepared cells in the freezer. 3. After the freezing cycle, transfer cells to liquid nitrogen.

Long-Term Storage

Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.


  • Contains DMSO, Glucose, Salts
  • Buffering capacity: pH = 7.2 to 7.6

Cytion’s Freeze Medium CM-ACF offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.

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