KATO-III Cells

Ham's F-12 Medium, also known as Ham's F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA).
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

Composition




Components
mg/L


Inorganic Salts
Calcium chloride x 2H2O
44,00



Copper(II) sulfate x 5H2O
0,00



Iron (II) sulfate x 7H2O
0,83



Magnesium chloride x 6H2O
122,00



Potassium chloride
223,65



Sodium chloride
7599,00



di-Sodium hydrogen phosphateanhydrous
142,04



Zinc sulfate x 7H2O
0,86


Other Components
D(+)-Glucose anhydrous
1801,60



Hypoxanthine
4,08



Linoleic acid
0,08



DL-α-Lipoic acid
0,21



Phenol red
1,20



Putrescine x 2HCl
0,16



Sodium pyruvate
110,00



Thymidine
0,73



NaHCO3
1176,00


Amino Acids
L-Alanine
8,91



L-Arginine x HCl
210,70



L-Asparagine x H2O
15,01



L-Aspartic acid
13,31



L-Cysteine x HCl x H2O
35,12



L-Alanyl-L-Glutamine
217,30



L-Glutamic acid
14,71



Glycine
7,51



L-Histidine x HCl x H2O
20,96



L-Isoleucine
3,94



L-Leucine
13,12



L-Lysine x HCl
36,54



L-Methionine
4,48



L-Phenylalanine
4,96



L-Proline
34,53



L-Serine
10,51



L-Threonine
11,91



L-Tryptophan
2,04



L-Tyrosine
5,44



L-Valine
11,71


Vitamins
D(+)-Biotin
0,01



D-Calcium pantothenate
0,24



Choline chloride
13,96



Folic acid
1,32



myo-Inositol
18,02



Nicotinamide
0,04



Pyridoxine x HCl
0,06



Riboflavin
0,04



Thiamine x HCl
0,34



Vitamin B12
1,36

Phosphate-Buffered Saline (PBS) Solution: The Optimal Buffer for Your Biological Research
Phosphate-buffered saline (PBS) is a versatile buffer solution used in many biological and chemical applications, as well as tissue processing. Our PBS solution is formulated with high-quality ingredients to ensure a constant pH during experiments. The osmolarity and ion concentrations of our PBS solution are matched to those of the human body, making it isotonic and non-toxic to most cells.
Composition of our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. We have chosen this composition based on CSHL protocols and Molecular cloning by Sambrook, which are well-established standards in the research community.
Applications of our PBS Solution
Our PBS solution is ideal for a wide range of applications in biological research. Its isotonic and non-toxic properties make it perfect for substance dilution and cell container rinsing. Our PBS solution with EDTA can also be used to disengage attached and clumped cells. However, it is important to note that divalent metals such as zinc cannot be added to PBS as this may result in precipitation. In such cases, Good's buffers are recommended. Moreover, our PBS solution has been shown to be an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, such as SARS-CoV-2.
Storage of our PBS Solution
Our PBS solution can be stored at room temperature, making it easy to use and access.
To sum up
In summary, our PBS solution is an essential component in many biological and chemical experiments. Its isotonic and non-toxic properties make it suitable for numerous applications, from cell culture to viral transport medium. By choosing our high-quality PBS solution, researchers can optimize their experiments and ensure accurate and reliable results.
Composition




Components
mg/L


Inorganic Salts
Potassium chloride
200,00



Potassium dihydrogen phosphate
200,00



Sodium chloride
8,000.00



di-Sodium hydrogen phosphate anhydrous
1,150.00