AS-30D Cells








General information
Description | Established in vitro from the AS-30D tumor ascites. |
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Organism | Rat |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | A-S-30D, AS30D |
Characteristics
Age | 16 months |
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Gender | Female |
Morphology | Round cells, loosely adherent, floating |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | AS-30D (Cytion catalog number 500116) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in Sprague-Dawley rats |
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Viruses | RAP-test: Negative. |
Karyotype | Hypodiploid rat karyotype with 12% tetraploidy, 38 (35-41). |
Handling
Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 26 hours |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | A ratio of 1:4 to 1:5 is recommended |
Seeding density | A seeding density of 1 x 10^6 cells/ml is recommended. |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D2Wox37: 150,152
Rat_D19Wox11: 228
Rat_D10Wox8: 266
Rat_D4Wox7: 153,157
Rat_D2Wox27: 211
Rat_D5Rat33: 122,124,128
Rat_D10Wox11: 156
Rat_D1Wox23: 210,214
Rat_D12Wox1: 410
Rat_D6Wox2: 104
Rat_D8Wox7: 182
Rat_D6Cebr1: 225
SRY: x,Y
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Required products
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.
Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:
8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)
This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.
Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Salts
Potassium chloride
200
Potassium phosphate monobasic anhydrous
200
Sodium chloride
8000
Sodium phosphate dibasic anhydrous
1150