WB-F344 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€375.00*

Prices excl. taxes plus shipping costs

Product number: 305201

Safety data sheet

Product sheet

Description	The WB-F344 rat liver epithelial cell line is a non-tumorigenic line extensively used in studies focusing on liver physiology, toxicology, and carcinogenesis. Originating from normal adult rat liver, these cells were initially derived to facilitate investigations into the mechanisms of liver regeneration and the bioactivation of chemical carcinogens in vitro. They are diploid, exhibiting stable karyotypic features that are characteristic of normal rat liver cells, making them a valuable model for genetic and cytological studies. WB-F344 cells are particularly noted for their ability to differentiate into bile duct-like structures in response to certain stimuli, which makes them an excellent tool for studying biliary epithelial function and pathology. Their robust response to growth factors and their ability to undergo oncogenic transformation under specific experimental conditions also provide a platform for exploring the molecular pathways involved in liver disease and cancer. Furthermore, these cells have been employed in studies assessing the hepatic toxicity of environmental and pharmaceutical compounds, providing critical insights into hepatocyte response to xenobiotic exposure. Due to their well-characterized nature and versatility in research applications, WB-F344 cells serve as a foundational model in hepatological research. Their use has contributed significantly to our understanding of liver biology, particularly in areas related to cell differentiation, carcinogenesis, and the hepatic response to injury and chemical insults.
Organism	Rat
Tissue	Liver
Synonyms	WB F344, WBF344

Age	Adult
Gender	Male
Morphology	Epithelial
Growth properties	Adherent

Citation	WB-F344 (Cytion catalog number 305201)
Biosafety level	1

Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

WB-F344 Cells

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA