TF-1 Cells








General information
Description | TF-1 cells are erythroblasts isolated from the bone marrow of a 35-year-old Asian male diagnosed with severe pancytopenia in 1987. These cells are a pivotal model for studying the complex processes of proliferation and differentiation within myeloid progenitor cells. As a cell line, TF-1 is heavily utilized in hematological research to understand the underlying mechanisms that govern cell cycle regulation and development in myeloid lineages. In addition to their primary role in hematopoietic research, TF-1 cells serve as a robust system for examining the impact of various cytokines on cell survival and growth. Their dependence on specific growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) for proliferation makes them an excellent tool for studying cytokine-mediated signaling pathways. This characteristic also makes TF-1 cells useful in evaluating the efficacy of new pharmacological agents that aim to modulate these pathways, thereby contributing significantly to therapeutic advances in treating myeloid disorders and other related diseases. |
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Organism | Human |
Tissue | Bone marrow |
Disease | Erythroleukemia |
Applications | The TF-1 cell line can be applied in various systems due to their responsiveness to multiple cytokines. They provide a good system to investigate the proliferation and differentiation of myeloid progenitor cells. Sensitive to GM-CSF, IL-3, EPO. |
Synonyms | TF1, MFD-1 |
Characteristics
Age | 35 years |
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Gender | Male |
Ethnicity | Japanese |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | TF-1 (Cytion catalog number 300434) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | TF-1 cells do not express glycophorin A or carbonyl anhydrase I. |
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Handling
Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS, for long-term culture: IL-3 |
Subculturing | Initiate cultures with a cell density of 2 x 10^5 cells/ml and maintain them within the range of 1 x 10^5 to 1 x 10^6 cells/ml. For subculturing, transfer the cell suspension to a fresh cell culture flask pre-filled with the correct volume of fresh culture medium. |
Seeding density | > 2 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
TH01: 7,9
TPOX: 8
vWA: 15,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 5,17
Penta D: 10,13
D8S1179: 11,15
FGA: 18,19
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HLA alleles |
A*: '02:01:01, '33:03:01
B*: '44:03:01, '51:01:01
C*: '01:02:01, '14:03:01
DRB1*: '09:01:02G, '13:02:01
DQA1*: '01:02:01, '03:02:01
DQB1*: '03:03:02, '06:04:01
DPB1*: '02:01:02, '04:01:01
E: '01:01:01, '01:03:01
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Required products
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.
Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:
8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)
This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.
Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Salts
Potassium chloride
200
Potassium phosphate monobasic anhydrous
200
Sodium chloride
8000
Sodium phosphate dibasic anhydrous
1150