LLC-PK1 Cells

€420.00*

Prices excl. taxes plus shipping costs

Product number: 607264

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	LLC-PK1 cells are a well-established and widely used cell line in biomedical research. These cells were derived from a healthy male pig's kidney, exhibiting typical epithelial morphology. The LLC-PK1 line is polarized and contains tight junctions, making it an ideal model for epithelial tissue. One of the critical features of LLC-PK1 cells is their ability to produce plasminogen activator, a substance that stimulates fibrinolysis. This property has made LLC-PK1 cells particularly valuable in thrombosis research. In recent years, plasminogen activator has been included in drugs used in thrombosis therapies since it facilitates the dissolution of small blood clots. In addition to producing plasminogen activators, LLC-PK1 cells produce large amounts of cytokeratin. This characteristic has made them popular for various pharmacologic and metabolic research investigations. The LLC-PK1 line has been used in drug metabolism, transport, toxicity, and interaction studies. LLC-PK1 cells are also frequently used in permeability assays. The mechanism of uracil transport differs depending on cell lines, with a Na+-independent system on the basolateral membrane in Caco-2 cells and both Na+-dependent and Na+-independent systems on the apical membrane in LLC-PK1 cells. Compared to other cell lines, LLC-PK1 cells share many characteristics of proximal tubular cells in vivo, including apical membrane microvilli, high activities of apical membrane enzymes, and expression of parathyroid hormone receptors and sodium-dependent glucose transporters. This makes LLC-PK1 cells a valuable tool in renal toxicology studies. Another cell line commonly used in renal toxicology studies is the MDCK cell line. Like LLC-PK1 cells, MDCK cells are epithelial but have characteristics more typical of distal tubular cells. They express vasopressin, oxytocin, and prostaglandin receptors, which, when stimulated, activate adenylate cyclase. LLC-PK1 and MDCK cell lines proliferate rapidly and can be passaged easily for many generations in monolayer cultures. LLC-PK1 cells are also capable of forming 'domes', fluid-filled blisters resulting from water and solute transport, tight junctions, and adhesion of the cells to the substratum. In conclusion, the LLC-PK1 cell line is a versatile and valuable tool for biomedical research. It has been widely used in various studies on drug metabolism, drug transport, drug toxicity, drug-drug interactions, renal toxicology, and permeability assays. With its well-established epithelial morphology and plasminogen activator and cytokeratin production, LLC-PK1 cells are an ideal model for epithelial tissue.
Organism	Sus Scrofa
Tissue	Kidney
Applications	Drug metabolism, permeability assays, toxicity, and interaction studies.
Synonyms	LLC-PK(1), LLC-PK-1, LLC PK-1, LLc-PK1, LLC PK1, LLCPK1, Lilly Laboratories Cell-Porcine Kidney 1

Breed/Subspecies	Hampshire
Age	3-4 weeks
Gender	Male
Morphology	Epithelial-like
Growth properties	Adherent/suspension. It takes a couple of days until cells grow in adherent colonies.

Citation	LLC-PK1 (Cytion catalog number 607264)
Biosafety level	The cell line contains Porcine type-C oncovirus (PCOV) sequences and transcripts. The infection mode is undetermined, and viral secretion cannot be excluded. In Germany, these viruses are classified as BSL 1 for humans and BSL 2 for animals (TRBA 462). However, the German Central Committee on Biological Safety (ZKBS) classifies these viruses and infected cell lines as BSL 2 for genetic modification applications.
NCBI_TaxID	9823
CellosaurusAccession	CVCL_0391

Viruses	Contains Porcine type-C oncovirus (PCOV) sequences and transcripts. Virus expression cannot be excluded.
Products	plasminogen activator

Culture Medium	Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820101a)
Supplements	Supplement the medium with 3% FBS
Dissociation Reagent	Accutase
Subculturing	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Seeding density	1 to 3 x 10⁶ cells/cm²
Fluid renewal	Every 3 days
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Required products

Required products

DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3

DMEM:Ham's F12 is a widely recognized and extensively utilized basal medium in cell culture for biological research. It serves as a fundamental source of nutrients for the growth of various mammalian cell lines, particularly when supplemented with Fetal Bovine Serum (FBS).

This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.

DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.

To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.

DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels.

Quality Control

Sterile-filtered

Storage and Shelf Life

Store at +2°C to +8°C, protected from light.
Once opened, store at 4°C and use within 6–8 weeks.

Shipping Conditions

Ambient temperature

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.

Composition

Category
Components
Concentration (mg/L)

Amino Acids
Glycine
18.75

L-Alanine
4.45

L-Arginine HCl
147.50

L-Asparagine H₂O
7.50

L-Aspartic Acid
6.65

L-Cysteine HCl H₂O
17.56

L-Cystine 2 HCl
31.29

L-Glutamic Acid
7.35

L-Glutamine
365.00

L-Histidine HCl H₂O
31.48

L-Isoleucine
54.47

L-Leucine
59.05

L-Lysine HCl
91.25

L-Methionine
17.24

L-Phenylalanine
35.48

L-Proline
17.25

L-Serine
26.25

L-Threonine
53.45

L-Tryptophan
9.02

L-Tyrosine Disodium Salt
48.10

L-Valine
52.85

Vitamins
D-Biotin
0.0035

Choline Chloride
8.98

D-Calcium Pantothenate
2.24

Folic Acid
2.66

myo-Inositol
12.60

Nicotinamide
2.02

Pyridoxine HCl
0.031

Pyridoxal HCl
2.00

Riboflavin
0.219

Thiamine HCl
2.17

Vitamin B12
0.68

Inorganic Salts
CaCl₂ 2 H₂O
154.50

CuSO₄ 5 H₂O
0.0013

Fe(NO₃)₃ 9 H₂O
0.05

FeSO₄ 7 H₂O
0.417

KCl
311.80

MgCl₂ 6 H₂O
61.20

MgSO₄
48.84

NaCl
6996.00

NaHCO₃
1200.00

Na₂HPO₄
71.02

NaH₂PO₄
54.30

ZnSO₄ 7 H₂O
0.432

Other Components
D-Glucose
3151.00

Hypoxanthine
2.40

HEPES
3574.50

Linoleic Acid
0.042

Lipoic Acid
0.105

Phenol Red Sodium Salt
8.63

Putrescine 2 HCl
0.081

Sodium Pyruvate
55.00

Thymidine
0.365

€25.00*

Accutase

Variants: 100 ml

Accutase Cell Dissociation Reagent
- A Gentle Alternative to Trypsin
Accutase is a cell detachment solution that is revolutionizing the cell culture industry. It is a mix of proteolytic and collagenolytic enzymes that mimics the action of trypsin and collagenase. Unlike trypsin, Accutase does not contain any mammalian or bacterial components and is much gentler on cells, making it an ideal solution for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. In this blog post, we will explore the benefits and uses of Accutase and how it is changing the game in cell culture.
Advantages of Accutase
Accutase has several advantages over traditional trypsin solutions. Firstly, it can be used whenever gentle and efficient detachment of any adherent cell line is needed, making it a direct replacement for trypsin. Secondly, Accutase works extremely well on embryonic and neuronal stem cells, and it has been shown to maintain the viability of these cells after passaging. Thirdly, Accutase preserves most epitopes for subsequent flow cytometry analysis, making it ideal for cell surface marker analysis.
Additionally, Accutase does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. This eliminates the need for an inactivation step and saves time for cell culture technicians. Finally, Accutase does not need to be aliquoted, and a bottle is stable in the refrigerator for 2 months.
Applications of Accutase
Accutase is a direct replacement for trypsin solution and can be used for the passaging of cell lines. Additionally, Accutase performs well when detaching cells for the analysis of many cell surface markers using flow cytometry and for cell sorting. Other downstream applications of Accutase treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Composition of Accutase
Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. It is formulated at a much lower concentration than trypsin and collagenase, making it less toxic and gentler, but just as effective.
Efficiency of Accutase
Accutase has been shown to be efficient in detaching primary and stem cells and maintaining high cell viability compared to animal origin enzymes such as trypsin. 100% of cells are recovered after 10 minutes, and there is no harm in leaving cells in Accutase for up to 45 minutes, thanks to autodigestion of Accutase.
In summary
In conclusion, Accutase is a powerful solution that is changing the game in cell culture. With its gentle nature, efficiency, and versatility, Accutase is the ideal alternative to trypsin. If you are looking for a reliable and efficient solution for cell detachment, Accutase is the solution for you.

€75.00*

PBS

Phosphate-Buffered Saline (PBS) Solution
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.

Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:

8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)

This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.

Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.

Quality Control

Sterile-filtered

Storage and Shelf Life

Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.

Shipping Conditions

Ambient temperature

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.

Composition

Category
Components
Concentration (mg/L)

Salts
Potassium chloride
200

Potassium phosphate monobasic anhydrous
200

Sodium chloride
8000

Sodium phosphate dibasic anhydrous
1150

€20.00*

Lot Number	Certificate Type	Date	Catalog Number
607264-030725	Certificate of Analysis	18. Aug. 2025	607264

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

LLC-PK1 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality control / Genetic profile / HLA

Certificate of Analysis (CoA)

Material Transfer Agreement