A Deep Dive into the Top 50 Most Sought-After Cell Lines in Our Portfolio

Our portfolio includes over 800 cell lines, crucial for biomedical research. Key cell lines like HeLa and HEK293T are highlighted for their widespread use and significant online search interest.

We've analyzed the top 50 cell lines from our portfolio, focusing on Google search popularity. This analysis helps identify current trends in cell line usage and anticipate future research directions.

Which are the most frequently searched cell lines?

The top 10 cell lines generate more than 230,000 Google searches every month. Including these, the top 50 cell lines from our collection receive nearly 300,000 monthly searches, highlighting the significant interest in the leading 10 research models detailed below.

The cell lines listed below are ranked by their monthly search volume:

Hela with a monthly search volume of 59,450.
HEK293T comes next with a volume of 46,450.
CHO has a search volume of 44,440.
HEK is searched 31,230 times a month.
THP-1 has a monthly volume of 17,400.
A549 is looked up 8,360 times.
Jurkat has a search volume of 7,730.
HepG2 is slightly lower at 6,600.
Hep 2 has a search volume of 6,400.
MCF7 has a monthly search volume of 4,870.

What sets the top 5 apart from the rest?

The top five cell lines garnering the most attention on Google, namely HeLa, HEK293T, CHO, HEK, and THP-1, are distinguished by several unique characteristics:

Hela

Origin: Originating from Henrietta Lacks' cervical cancer cells in 1951, HeLa cells are among the first and most extensively researched immortal cell lines.
Immortality: Their ability to divide indefinitely makes them invaluable for prolonged studies and large-scale cultures.
Adaptability: HeLa cells can thrive in various culture conditions, showcasing remarkable resilience.

HEK293T

Transfection Proficiency: Known for their high transfection efficiency, these cells are optimal for introducing foreign DNA and expressing recombinant proteins.
Viral Vector Support: Their capability to efficiently replicate viral vectors makes them ideal for generating viral-based gene delivery systems.
Genetic Flexibility: The ease of genetic modification in HEK293T cells facilitates the creation of stable cell lines for specific research needs.

CHO

Protein Production: CHO cells excel in generating complex proteins with accurate folding and modifications, crucial for biopharmaceuticals.
Mammalian System: As mammalian cells, CHO cells perform human-like post-translational modifications, ensuring biologically active protein therapeutics.
Scale-Up Capacity: Their ability to grow in high-density suspension cultures makes them suitable for large-scale recombinant protein manufacturing.

HEK

Research Versatility: HEK cells are adaptable to various research applications, including protein studies, transfection experiments, and more.
Wide Availability: Easily obtainable from various sources, HEK cells are accessible to researchers globally.
Experimental Flexibility: Compatible with numerous transfection and culture techniques, HEK cells support diverse experimental setups.

THP-1

Monocytic Lineage: Originating from human acute monocytic leukemia, THP-1 cells serve as a model for monocyte/macrophage studies and immune research.
Differentiation Potential: Treatable with agents like phorbol esters to differentiate into macrophage-like cells, THP-1 offers insights into mature monocyte functions.
Disease Model: THP-1 cells are instrumental in researching conditions involving monocyte/macrophage roles, such as in cancer, infectious diseases, and autoimmune disorders.

These cell lines' unique properties underpin their widespread application in biomedical research, proving essential for exploring basic biology, disease mechanisms, and potential treatments.

Do HeLa Cells Truly Dominate the Realm of Scientific Importance?

While HeLa cells frequently top the charts in online searches, possibly due to their entanglement in legal battles, documentaries, and literature, does this spotlight reflect their actual prominence in scientific research? An analysis by CiteAB suggests a different hierarchy based on scientific citations, where HeLa cells, despite their search engine fame, rank fourth with a citation share of 3.10%. Surprisingly, the crown is held by HEK293T cells, boasting a citation rate of 6.22%. This revelation, along with the notable presence of other cell lines like Vero C1008, Vero, and A-549 in research citations, paints a complex picture.

Top 50 Cell Lines from Our Portfolio with the Highest Google Search Volumes

No	Cell Line	Description
1	Hela	A robust and widely used immortal cell line derived from cervical cancer, invaluable for studying cancer biology and virology.
2	HEK293T	Human embryonic kidney cells transformed with the SV40 large T antigen, popular for protein expression and viral vector production.
3	CHO	Chinese hamster ovary cells, frequently utilized in biopharmaceutical production due to their capacity for high-level recombinant protein expression.
4	HEK	Human embryonic kidney cells, versatile in various research applications including transfection studies and protein expression.
5	THP-1	Human acute monocytic leukemia cells, commonly employed in immunology and inflammation research.
6	A549	Human lung carcinoma cells, extensively used in respiratory disease studies and cancer research.
7	Jurkat	Human T lymphocyte cells, crucial for immunological and leukemia research, particularly in apoptosis and T cell signaling.
8	HepG2	Human hepatocellular carcinoma cells, valuable for liver-related studies including drug metabolism and hepatitis research.
9	Hep 2	Epithelial cells derived from laryngeal carcinoma, often used in virology and molecular biology research.
10	MCF7	Human breast adenocarcinoma cells, a staple in breast cancer research and endocrine therapy studies.
11	Cos-7	African green monkey kidney fibroblast-like cells, commonly used for transient transfection studies.
12	Caco-2	Human colorectal adenocarcinoma cells, widely employed in drug transport and absorption studies.
13	NIH 3T3	Mouse embryonic fibroblast cells, frequently utilized in cell biology, oncology, and stem cell research.
14	HCT116	Human colorectal carcinoma cells, extensively studied for cancer genetics and drug sensitivity testing.
15	Vero	African green monkey kidney cells, popular for viral vaccine production and virology research.
16	BHK-21 clone 13	Baby hamster kidney cells, utilized in virology, molecular biology, and vaccine production.
17	PC-12	Rat adrenal pheochromocytoma cells, commonly used in neuroscience research, particularly in studies related to neuronal differentiation and signaling.
18	3T3-L1	Mouse embryonic fibroblast preadipocytes, crucial for adipogenesis and obesity research.
19	MRC-5	Human fetal lung fibroblast cells, widely used in vaccine development, virology, and cytotoxicity assays.
20	HaCaT	Immortalized human keratinocytes, important for dermatological and wound healing studies.
21	Raji	Human B lymphoblastoid cells, commonly utilized in immunology, virology, and cancer research.
22	SH-SY5Y	Human neuroblastoma cells, frequently employed in neuroscience and neuropharmacology research.
23	PC-3	Human prostate cancer cells, extensively used in prostate cancer biology and drug discovery.
24	U2OS	Human osteosarcoma cells, valuable for studying osteogenesis and cancer biology.
25	Huh-7	Human hepatocellular carcinoma cells, important for hepatitis C virus research and liver disease modeling.
26	K562	Human chronic myelogenous leukemia cells, widely studied in hematological research, particularly in erythropoiesis and leukemia.
27	U87MG	Human glioblastoma cells, commonly used in neuro-oncology research and drug screening.
28	Neuro2a	Mouse neuroblastoma cells, frequently employed in neuroscience and neurotoxicology research.
29	HL-60	Human promyelocytic leukemia cells, crucial for hematopoietic and leukemia research, particularly in differentiation studies.
30	HT29	Human colorectal adenocarcinoma cells, extensively used in cancer biology and drug development, especially in studies related to colon cancer.
31	MDCK (NBL-2)	Madin-Darby canine kidney cells, commonly used in virology and epithelial transport studies.
32	U937	Human histiocytic lymphoma cells, important for immunology and cancer research, particularly in monocyte/macrophage differentiation studies.
33	C2C12	Mouse myoblast cells, essential for skeletal muscle research and myogenesis studies.
34	A375	Human melanoma cells, widely used in melanoma biology and drug screening.
35	MV4-11	Human acute myeloid leukemia cells, frequently employed in hematological research, particularly in AML drug discovery.
36	MCF10A	Human mammary epithelial cells, crucial for breast cancer and mammary gland biology research.
37	Nalm-6	Human pre-B acute lymphoblastic leukemia cells, important for hematological and leukemia research.
38	HT1080	Human fibrosarcoma cells, widely used in cancer biology, angiogenesis, and metastasis research.
39	ARPE-19	Human retinal pigment epithelial cells, important for ocular biology and retinal disease research.
40	AsPC-1	Human pancreatic adenocarcinoma cells, valuable for pancreatic cancer and drug sensitivity studies.
41	Mia PaCa-2	Human pancreatic adenocarcinoma cells, commonly used in pancreatic cancer research and drug discovery.
42	NCI-H1299	Human non-small cell lung carcinoma cells, crucial for lung cancer biology and drug screening.
43	L929	Mouse fibroblast cells, frequently utilized in cytotoxicity assays and vaccine production.
44	B16	Mouse melanoma cells, important for melanoma biology and immunotherapy research.
45	Daudi	Human Burkitt's lymphoma cells, commonly used in immunology and cancer research, particularly in B cell signaling studies.
46	EL-4	Mouse T lymphoma cells, crucial for immunology and cancer research, especially in studies related to T cell activation and regulation.
47	T47D	Human breast ductal carcinoma cells, extensively studied in breast cancer biology and hormone receptor research.
48	Caki-1	Human clear cell renal cell carcinoma cells, important for renal cancer research and drug sensitivity testing.
49	Calu-1	Human non-small cell lung carcinoma cells, commonly used in lung cancer research and drug development.
50	HEK293T/17	A variant of HEK293T cells, popular for protein expression and viral vector production.