MCF10A Cells
Overview of the MCF 10A cell line
Description | The MCF10A human mammary epithelial cell line, established from the mammary gland of a 36-year-old female with fibrocystic disease, serves as a model for studying the intricacies of normal breast cell function, transformation, and the epithelial to mesenchymal transition critical in invasive breast carcinoma transition. As a non-tumorigenic epithelial cell line derived from benign proliferative breast tissue, MCF10A cells are instrumental in mammary cell studies, offering insights into breast tumor progression and the dynamics of tumor cells in mammospheres. MCF10 A cells, characterized by their three-dimensional growth in collagen and their ability to form acinar structures in mixed Matrigel, provide a reliable model for analyzing the impact of oncogenes and studying the mammosphere formation, which is crucial for understanding the properties of mammary progenitor cells and their role in cancer research. The MCF10A cell line, while exhibiting a basal-like phenotype, express a combination of luminal and stem-like markers, as well as epithelial-cell markers such as cytokeratins and milk proteins. Their responsiveness to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF) underscores the importance of growth factors and hormones in the proliferation and survival of human breast tissue cells. The MCF 10A model, provides a window into the genomic signaling pathways that govern cell behavior and phenotype in 3D culture, offering a platform for immunohistochemistry and immunofluorescence staining to visualize cellular processes. |
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Organism | Human |
Tissue | Mammary gland, breast |
Synonyms | MCF-10A, MCF 10A, MCF.10A, MCF10A, MCF10-A, MCF10a, MCF-10 Attached, Michigan Cancer Foundation-10A |
Aspects of MCF 10A epithelial cells
Age | 36 years |
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Gender | Female |
Morphology | Epithelial |
Growth properties | Adherent |
Documentation
Citation | MCF 10A (Cytion catalog number 305026) |
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Biosafety level | 1 |
Genomics of the human breast cancer cell line MCF 10A
Tumorigenic | No |
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Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 5% horse serum, 20 ng/mL EGF, 0,5 microgram/mL Hydrocortison, 10 microgram/mL Insulin. Add 100 ng/mL cholera toxin if needed. |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Genetic profile of MCF10A cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
TH01: 8,9.3
TPOX: 9,11
vWA: 15,17
D3S1358: 14,18
D21S11: 28,3
D18S51: 18,19
Penta E: 13,14
Penta D: 10,12
D8S1179: 14,16
FGA: 22,24
D6S1043: 12,18
D2S1338: 21,26
D12S391: 17,2
D19S433: 13,15
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