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Cell Banking Service

Ensuring Reliable and High-Quality Research-Grade Cell Lines

At Cytion, we are dedicated to providing researchers with top-tier, research-grade cell lines through our premier cell banking service.

Our focus is on the meticulous manufacturing of high-quality vials, ensuring each batch meets the stringent standards required for scientific research.

Trust in Cytion to supply reliable cell culture solutions, supporting your research endeavors with confidence and precision.

Types of Cell Banks

At Cytion, we offer a comprehensive suite of research-grade cell banking options, each tailored to meet the diverse needs of research and development, from foundational research cell banks to end-of-production quality checks.

1. Research Cell Bank (RCB)

This cell bank acts as a foundation for the development of a master cell bank. As the name suggests, it is used for research and development purposes. It's a pre-GMP cell bank, meaning regulations are not as stringent compared with those for GMP-developed banks.

2. Master Cell Bank (MCB)

This is created using a well-characterized monoclonal cell line. To ensure the identity and purity of cell lines, master cell bank characterization is performed using assays, such as:

  • Mycoplasma and retroviral reverse transcriptase activity tests
  • In vitro and in vivo safety testing assays
  • Screening tests

The MCB is developed after the RCB.

3. Working Cell Bank (WCB)

This is formed from the continued subculture of the MCB. WCBs are used to maintain a consistent supply of cells for experimentation or production purposes. It is important to maintain the identity, genetic stability, and viability of the cells in the WCB to ensure reproducibility of results and consistency in product quality.

Quality control measures include:

  • Monitoring WCB characteristics
  • Ensuring regulatory requirements are met

4. End-of-Production Cell Bank (EOP CB)

This cell bank is created from vials of the working cell bank (WCB). The cell bank undergoes detailed analysis, including assessments of:

  • Cell count and viability
  • Cell shape and genetic structure (karyotyping)
  • Presence of contaminants like mycoplasma
  • Existence of endotoxins

These cells act as a quality check for future use in research and development and can serve as references.

From Concept to Cell: Custom Cell Banking Solutions

At Cytion, we offer extensive customization and characterization options to meet your high-quality cell banking needs. Our comprehensive services ensure the reliability and authenticity of your cell lines, providing tailored solutions for various research applications.

Customization for Cell Banking Services

We offer extensive customization and characterization options for your high-quality cell banking needs, including:

  • Vial Sizes: Volumes range from 250 µl to 5 ml, tailored to your specific requirements.
  • Cell Numbers: Customizable from 0.1 million to 50 million cells per milliliter, ensuring flexibility for various research applications.
  • Functional Tests: Evaluation of cell viability, growth, and response to stimuli to ensure optimal performance and reliability.
  • Contamination Checks: Rigorous testing to confirm the absence of species-specific pathogens.
  • Authentication: Comprehensive verification of cell identity to guarantee the reproducibility of your cell lines.

Process for Establishing Your Cell Bank

  1. Consultation: A detailed consultation to understand your specific research and cell banking needs.

  2. Integration: Transfer your cells to us or select from our collection of high-quality, genetically authentic cell lines.

  3. Production: We produce cryopreserved cells, ensuring consistent, high-quality cell samples.

  4. Transfer: Cells are delivered in temperature-monitored dry shippers, ensuring safe and controlled delivery of up to 500 vials per shipment. Vials are transported in cryoboxes, protecting labels and contents.

Service Details

  1. Automated Filling Device: Utilizing the Fill-It automated filling device (Sartorius Stedim Biotech), we produce large quantities of cryovials, ensuring precision and consistency far superior to manual methods.

  2. Controlled-Rate Freezing: Employing the CryoMed™ controlled-rate freezer (Thermo Fisher Scientific), we achieve optimal cryopreservation with a cooling rate of 1°C per minute, maintaining cell integrity and functionality.

  3. Cell Culture Vessels for Large-Scale Expansion: We utilize a range of cell culture flasks to support large-scale cell expansion, including CellSTACKs®, CellFactory™ devices (Nunc™), and CELLdisc™ (Greiner Bio-One).

  4. Long-Term Storage: Cryopreserved cells are stored in temperature-monitored tanks in the vapor phase of liquid nitrogen, ensuring high-quality cells are available for immediate use post-thaw for many years.

Benefits of Cytion Cell Banking

1. Ensuring Reproducibility and Consistency

  • Standardized Cell Lines: Maintain genetic and phenotypic characteristics.
  • Reliable Results: Minimize experimental variability and enhance reproducibility.

2. Reducing Time and Costs

  • Efficient Process: Streamline cell line development and reduce time to market.
  • Cost Savings: Avoid repetitive cell isolation and validation steps.

3. Supporting Research and Clinical Trials

  • Readily Available Cells: Quick access to authenticated cell lines for studies and trials.
  • Regulatory Compliance: Use standardized and validated cells to support regulatory submissions.

4. Enhancing Scalability

  • Optimized Bioprocessing: Use working cell banks for efficient seed train intensification.
  • Streamlined Production: Facilitate the transition from small-scale cultures to large-scale batches within weeks.

Contact Us to Facilitate Your Cell Banking

For more information on our custom Cell Banking Services, please contact us. Our team of experts is ready to discuss your needs and provide a solution tailored to your research goals.

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