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Assay-Ready Cell Service 

Our assay-ready cell service offers customizable solutions tailored to your research needs, with vial sizes ranging from 1 to 5 ml and cell numbers from 1 to 100 million cells per milliliter. We conduct functional tests to assess cell viability, growth, and response to stimuli, ensuring assay suitability, and perform rigorous contamination checks and authentication to verify cell identity for experimental reliability.

Our service offers researchers unparalleled efficiency and consistency by providing high-quality, ready-to-use mammalian cell lines that perform immediately post-thaw, streamlining experimental preparation and ensuring reproducible results.

Advantages of Our Assay-Ready Cell Manufacturing Service

1. Time and Resource Efficiency

Our services streamline experimental preparation by eliminating extensive maintenance, subculturing, and growth optimization for our clients. This allows researchers to focus on value-adding activities and accelerates the experimental process.

2. Consistency and Reduced Variability

Cytion's assay-ready cell service ensures a consistent and reproducible experimental setup. This standardization reduces experimental variability and facilitates accurate comparisons across studies and laboratories.

3. Standardized Volumes and Concentrations

Our assay-ready cells, banked at standardized volumes and concentrations, ensure the integrity of your experiments and enhance reproducibility.

Customization and Characterization

We offer customization and characterization options for your high-quality assay-ready cells, including:

  • Vial Sizes: Volumes range from 1 ml to 5 ml, depending on experimental needs.
  • Cell Numbers: Customizable from 1 million to 100 million cells per milliliter.
  • Functional Tests: Assess cell viability, growth, and response to stimuli to confirm their suitability for assays.
  • Contamination Checks: Rigorous testing to ensure cells are free from species-specific pathogens.
  • Authentication: Verification of cell identity and genetic stability to ensure experimental reliability.
  • Shipping: Temperature-monitored dry shippers capable of holding up to 500 vials, ensuring the safe and controlled delivery of your assay-ready cells.

From Concept to Cell: Custom Assay-Ready Solutions

Process for Getting Your Personalized Assay-Ready Cells

1. Consultation: We understand your research needs through a detailed consultation.

2. Integration: Transfer your cells to us or choose from our high-quality, genetically authentic cell lines.

3. Production: We produce cryopreserved, assay-ready cells, ensuring consistent and reliable results.

4. Transfer: We deliver the cells to you in a temperature-monitored dry shipper, capable of holding up to 500 vials per shipment.
The vials are transported in cryoboxes, not straws, ensuring the labels and contents remain undamaged.

Applications in Bioassays and Drug Discovery


Bioassays for Biologics Quality Control

Quality control of therapeutic proteins, antibodies, and vaccines requires robust, precise bioassays. Our assay-ready cells maintain consistent performance across different testing sites, improving inter-laboratory precision and facilitating long-term bioassay support.


Drug Discovery and High-Throughput Screening (HTS)

For cell-based HTS, large quantities of consistent cells are essential to avoid costly delays. Our assay-ready cells, tested to meet assay specifications, enable the timely separation of cell preparation from screening, enhancing the efficiency of drug discovery campaigns. Custom cell banks from recombinant or non-recombinant lines expressing targets of interest are available to support various research projects.

Service Details


  • Automated Filling Device: Cytion utilizes the Fill-It automated filling device (Sartorius Stedim Biotech) to produce large quantities of cryovials in an assay-ready format. This technology ensures precision and consistency that surpasses manual production.
  • Cell Counting with Vi-CELL BLU: For cell counting and viability analysis, we use the Vi-CELL BLU. The Vi-CELL BLU captures up to 100 images per 200 µl sample and supports a cell concentration range of 50,000 to 15,000,000 cells/mL, ensuring precise, consistent results. It also complies with 21 CFR Part 11 requirements for regulatory support.
  • Controlled-Rate Freezing: Optimal cryopreservation is achieved using the CryoMed™ controlled-rate freezer. The controlled-rate freezing ensures a cooling rate of 1°C per minute for the cell suspension, maintaining cell integrity and functionality.
  • Cell Culture Vessels Supporting Large-Scale Expansion: We employ a variety of cell culture flasks to support large-scale cell expansion, including CellSTACKs®, CellFactory™ devices (Nunc™), and CELLdisc™ (Greiner Bio-One).
  • Long-Term Storage: Assay-ready cells are stored in temperature-monitored tanks in the vapor phase of liquid nitrogen, guaranteeing high-quality cells for many years, ready for immediate use post-thaw.

Ready to elevate your research with Assay-Ready Cells?

Contact us to enhance your research efficiency and ensure reproducibility with our Assay-Ready Cell Service.

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Frequently Asked Questions about Assay-Ready Cells

Assay-ready cells are pre-prepared, high-quality mammalian cell lines designed to be used immediately after thawing, without requiring a recovery period. These cells are meticulously prepared to function as reagents, ensuring optimal performance in various assays and experiments.
  • Time and resource efficiency: Assay-ready cells eliminate the need for extensive cell maintenance and growth optimization.
  • Consistent and reproducible experimental setups: These cells reduce variability in experiments.
  • Enhanced data quality: The reliability of assay-ready cells leads to improved data quality.
  • Minimized contamination risk: Standardized banking and handling procedures reduce the risk of contamination.
Assay-ready cells are essential in pharmaceutical research, cosmetics, and food industries. They are used for identifying functional ingredients, screening chemical or biological entities, and verifying the biological function of therapeutic proteins and antibodies.
  • Functional tests: Assess cell viability, growth, and response to stimuli.
  • Rigorous contamination checks: Ensure cells are free from species-specific pathogens.
  • Authentication: Verify cell identity and genetic stability.
Yes, Cytion customizes assay-ready cells based on client specifications, including vial sizes (250 µl to 5 ml), cell numbers (2 million to 50 million cells per vial), and storage solutions (Master and Working Cell Banks).
Cytion uses the Fill-It automated filling device (Sartorius Stedim Biotech) for precision and consistency in producing large quantities of cryovials. This surpasses manual production, ensuring the highest quality and reliability.
Cytion employs the CryoMed™ controlled-rate freezer (Thermo Fisher Scientific), which maintains a precise freezing rate of 1°C per minute. This process preserves cell integrity and functionality.
Cytion uses various cell culture flasks, including CellSTACKs®, CellFactory™ devices (Nunc™), and CELLdisc™ (Greiner Bio-One). Each type offers unique advantages for efficient cell growth and large-scale expansion.
Assay-ready cells are stored in temperature-monitored tanks in the vapor phase of liquid nitrogen. They are shipped using temperature-monitored dry shippers capable of holding up to 500 vials, ensuring safe and controlled delivery.
Assay-ready cells provide large quantities of consistent cells essential for HTS, avoiding costly delays. They enable the separation of cell preparation from screening, enhancing the efficiency of drug discovery campaigns.
In quality control of therapeutic proteins, antibodies, and vaccines, assay-ready cells ensure consistent performance across testing sites. This improves inter-laboratory precision and supports long-term bioassay reliability.
Cytion combines advanced technology, meticulous quality control, and customizable services to meet diverse research needs. Our assay-ready cells offer reliable, reproducible results, empowering researchers to focus on their experiments with confidence. Experience the revolution in cell culture with Cytion.
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