KG-1 Cells


General information
Description | KG-1 is a human acute myelogenous leukemia (AML) cell line derived from the bone marrow of an adult patient with erythroleukemia. This cell line is a valuable model for studying hematopoietic differentiation and leukemia, particularly due to its unique characteristics, including the expression of several hematopoietic markers. KG-1 cells are classified as immature myeloid cells that resemble early progenitor cells, which makes them a useful tool for investigating the early stages of myeloid lineage commitment and the molecular mechanisms driving leukemogenesis. KG-1 cells exhibit a high degree of plasticity, which allows them to differentiate into various hematopoietic lineages under the right experimental conditions. This characteristic is particularly important for research in understanding the regulation of hematopoiesis and the development of therapeutic strategies aimed at targeting leukemic stem cells. Additionally, KG-1 cells are known to express markers such as CD34, HLA-DR, and CD13, which are critical in both normal and malignant hematopoiesis, making them an excellent model for flow cytometry and other immunophenotyping studies. KG-1 has also been employed in drug discovery and toxicity testing, where its responsiveness to differentiation agents and chemotherapeutic drugs can be evaluated. As with all in vitro models, it is important to recognize that KG-1 cells are for research use only and not suitable for therapeutic or in vivo applications. |
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Organism | Human |
Tissue | Bone marrow |
Disease | Acute myelogenous leukemia |
Synonyms | KG1 |
Characteristics
Age | 59 years |
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Gender | Male |
Ethnicity | Caucasian |
Cell type | Myeloblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | KG-1 (Cytion catalog number 300208) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | HLA A30, A31, B35, Cw4 |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 0, ES-D, 1, Me-2, 1, AK-1, 0, GLO-1, 2 |
Viruses | EBNA (EBNA ): negative |
Reverse transcriptase | Negative |
Handling
Culture Medium | IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 45 hours |
Subculturing | Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 - 3 x 10^5 cells/ml. Split the cells when a maximum cell density of 1 - 2 x 10^6 cells/ml is reached. |
Split ratio | A ratio of 1:2 is recommended |
Fluid renewal | Every 3 days |
Freezing recovery | Allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 7
D13S317: 11,12
D16S539: 11
D5S818: 13
D7S820: 8,10
TH01: 7,8
TPOX: 7,9
vWA: 14,19
D3S1358: 15,16
D21S11: 28,29
D18S51: 10.2,18
Penta E: 7,13
Penta D: 8,9
D8S1179: 13,14
FGA: 22
PEZ6: HepG2
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Required products
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.
Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:
8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)
This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.
Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Salts
Potassium chloride
200
Potassium phosphate monobasic anhydrous
200
Sodium chloride
8000
Sodium phosphate dibasic anhydrous
1150
Certificate of Analysis (CoA)
Lot Number | Certificate Type | Date | Catalog Number |
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300208-121 | Certificate of Analysis | 15. Apr. 2025 | 300208 |