FRhK-4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€550.00*

Prices excl. taxes plus shipping costs

Product number: 305151

Safety data sheet

Product sheet

Description	The FRhK-4 cell line consists of fibroblast-like cells derived from the kidney of a fetal rhesus monkey (Macaca mulatta). This cell line is widely used in biomedical research due to its relevance to primate biology and its utility in studying viral infections, nephrotoxicity, and renal physiology. The cells exhibit typical fibroblast morphology, characterized by an elongated shape and a branching architecture, which facilitates numerous types of cell and molecular biology experiments. FRhK-4 cells are particularly noted for their susceptibility to various viruses, including simian virus 40 (SV40) and polyomavirus. This makes them an excellent model for studying viral mechanisms of infection, replication, and oncogenesis in a primate system. Additionally, their origin from kidney tissue allows researchers to explore cellular responses to renal toxins and drugs, making them a valuable tool for pharmacological studies and toxicity assessments. Moreover, the genetic and physiological similarities of the FRhK-4 cells to human cells support their use in translational research, where findings may have direct implications for understanding human kidney diseases and developing therapeutic strategies. The use of this cell line in diverse research settings underscores its versatility and importance in scientific studies that require a non-human primate model.
Organism	Rhesus macaque
Tissue	Embryonic kidney
Synonyms	FRHK-4, Frhk-4, FRhK4, Fetal Rhesus Kidney-4

Age	Fetus
Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Citation	FRhK-4 (Cytion catalog number 305151)
Biosafety level	1

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

FRhK-4 Cells

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA