DMS-79 Cells

€550.00*

Content: 1 cryovial

Prices excl. taxes plus shipping costs

Product number: 300164

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	DMS-79 is a human lung cancer cell line derived from a small cell lung carcinoma. These cells exhibit a classical neuroendocrine phenotype, which is characteristic of small cell lung cancer. This phenotype is significant because it implies a potential utility in studying neuroendocrine signaling pathways, which are crucial in the development and progression of lung cancer. The DMS-79 cell line has been widely utilized in research to understand the molecular biology of lung cancers, particularly in the context of tumor genesis, cell proliferation, and apoptosis. The cell line is known for its aggressive growth and high tumorigenicity in vivo, making it an excellent model for in vivo studies of tumor behavior and response to therapeutics. DMS-79 cells also serve as a useful tool for pharmacological testing and drug development, offering insights into the cellular responses to various chemotherapeutic agents. Furthermore, these cells have been instrumental in the study of cancer stem cell characteristics and mechanisms of metastasis in small cell lung carcinoma. This extensive usage underscores the importance of DMS-79 in cancer research, particularly in therapies targeting aggressive and hard-to-treat cancers like small cell lung carcinoma.
Organism	Human
Tissue	Lung
Disease	Carcinoma, azaserine induced
Metastatic site	Pleural effusion
Synonyms	DMS 79, DMS79

Age	65 years
Gender	Male
Ethnicity	Caucasian
Growth properties	Aggregates in suspension

Citation	DMS-79 (Cytion catalog number 300164)
Biosafety level	1

Receptors expressed	Epidermal growth factor (EGF)
Antigen expression	Leu 7, My23, Class 1 HLA, Class 2 HLA
Oncogenes	c-myc +, N-myc +, c-raf-1 +, Ha-ras +, Ki-ras +, N-ras +, v-fes -, v-fms -
Tumorigenic	Yes, in nude mice
Products	Adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, calcitonin, corticotropin, beta endorphin, 17 beta estradiol, lipotropin, oxytocin - neurophysin (OT-NP), parathormone, somatostatin-like immunoreactivity (SRIF)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium supplements	Supplement the medium with 10% heat-inactivated FBS, add 2.5 g/L glucose and 10 mM HEPES
Doubling time	96 hours
Subculturing	Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Subculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate at 37 degree Celsius for 10minutes. Collect the cells following centrifugation, resuspend in fresh cell culture medium and count. Start cultures at 2-4 x 10^4 cells/ml.
Split ratio	A ratio of 1:2 to 1:4 is recommended
Seeding density	2 to 4 x 10^4 cells/cm^2
Fluid renewal	2 to 3 times per week
Freezing recovery	After thawing, allow the cells to recover from the freezing process for at least 24 hours.
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,y CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 10 D7S820: 9,11 TH01: 8 TPOX: 8 vWA: 18 D3S1358: 8 D21S11: 30 D18S51: 14,17 Penta E: 7 Penta D: 11,13 D8S1179: 12,14 FGA: 21
HLA alleles	A: '01:01:01, '02:01:01 B: '08:01:01, '35:01:01 C: '04:01:01, '07:01:01 DRB1: '11:01:01, '14:01:01 DQA1: '01:04:01, '05:05:01 DQB1: '03:01:01, '05:03:01 DPB1*: '03:01:01, '10:01:01 E: '01:01, '01:03

Required products

PBS

Phosphate-Buffered Saline (PBS) Solution
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.

Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:

8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)

This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.

Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.

Quality Control

Sterile-filtered

Storage and Shelf Life

Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.

Shipping Conditions

Ambient temperature

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.

Composition

Category
Components
Concentration (mg/L)

Salts
Potassium chloride
200

Potassium phosphate monobasic anhydrous
200

Sodium chloride
8000

Sodium phosphate dibasic anhydrous
1150

€20.00*

Certificate of Analysis (CoA)

Lot Number	Certificate Type	Date	Catalog Number
300164-913	Certificate of Analysis	23. May. 2025	300164

DMS-79 Cells

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA

Certificate of Analysis (CoA)