SK-OV-3 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300342

安全資料表

產品說明書

分析證明書 (CoA)

說明	SK-OV-3 cells, also known as SKOV3 cells, were derived from the ascitic fluid of a 64-year-old Caucasian female with ovarian cancer, are used in the study of serous cystadenocarcinoma, a subtype of ovarian carcinoma. These cells are known for their resistance to tumor necrosis factor and various cytotoxic drugs, including cisplatin, highlighting the challenges in chemotherapy for ovarian cancer treatment and makes them an excellent model for studying the mechanisms underlying cisplatin resistance and exploring new therapeutic strategies. The antioxidant system, including the thioredoxin antioxidant system (Trx), plays a crucial role in the survival and resistance of SK-OV-3 cells, offering a target for interventions aimed at sensitizing cancer cells to chemotherapy. The use of compounds like quercetin to modulate the antioxidant system and induce apoptosis in SK-OV-3 cells highlights the potential for dietary antioxidants in cancer therapy. In addition to their role in studying drug resistance, SK-OV-3 cells are used to investigate the invasive behavior of ovarian carcinoma cells and the interaction between cancer cells and the tumor microenvironment, including the role of M0 and M2 macrophages in tumor progression. The application of SK-OV-3 cells in cancer research extends to the development of xenograft models and the use of reporter genes, such as firefly-Luc, to monitor tumor growth and metastasis in vivo. Overall, SK-OV-3 cells serve as a critical model for understanding the complexity of ovarian cancer, from the molecular mechanisms driving resistance and estrogen signaling to the interaction between cancer cells and the tumor microenvironment.
生物體	Human
組織	Ovary
疾病	Serous cystadenocarcinoma
轉移部位	Ascites
同義詞	SKOV-3, SK-OV3, SK.OV.3, SKOV3, Skov3, SKO3

年齡	64 years
性別	Female
族裔	Caucasian
生長特性	Adherent

引用	SK-OV-3 (Cytion catalog number 300342)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0532

同工酶	PGM3, 1, PGM1, 1-2, ES-D, 1, Me-2, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0311
致瘤性	Forms moderately well differentiated adenocarcinoma consistent with ovarian primary
核型分析	(P16) hypodiploid to hypotetraploid with dicentrics and large telocentric

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm²
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300342-1420SF	分析證明書	23. May. 2025	300342
300342-220425	分析證明書	23. May. 2025	300342
300342-131125	分析證明書	08. Jan. 2026	300342

SK-OV-3 Cells

Key points about the SK-OV-3 cell line

Properties of ovarian cancer cell line SKOV3

Documentation

Genomics

Handling SKOV3 cells

Genetic profile

分析證明書 (CoA)

生物體	Human
組織	Breast, mammary gland
疾病	Invasive ductal carcinoma

生物體	Human
組織	Lung
疾病	Adenocarcinoma (grade III)