SK-MEL-28 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300337

安全資料表

產品說明書

分析證明書 (CoA)

說明	This cell line was established from an axillary lymph node of a 51-year-old male of unknown ethnicity by T.Takahashi and associates who have isolated this cell line in a series of melanoma lines.
生物體	Human
組織	Skin
疾病	Cutaneous melanoma
同義詞	SK-Mel-28, SK.MEL.28, SK-MEL 28, SK MEL-28, SK MEL 28, SK Mel 28, SKMel-28, SKMEL-28, SK-MEL28, SK-Mel28, SK Mel28, SKMEL28, SKMel28, SKmel28, SKML-28, SK28, AU-Mel, P-36, P36

年齡	51 years
性別	Male
形態學	Polygonal
生長特性	Adherent

引用	SK-MEL-28 (Cytion catalog number 300337)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0526

蛋白質表達	P53 positive
同工酶	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
致瘤性	Yes, in nude mice. Forms malignant melanoma (large round cell type)
產品	Melanin
突變譜	BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
流體更新	2 to 3 times per week
解凍後的恢復	Leave at least 48 hours post to thawing until removal of medium or subculture
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300337-1315SF	分析證明書	23. May. 2025	300337
300337-130125	分析證明書	23. May. 2025	300337

SK-MEL-28 Cells

Key points about the SK-MEL-28 cell line

Details

Specifications

Genetic profile of the human melanoma cell line SK-MEL-28

Maintenance techniques

SKMEL28 cell purity and identity checks

分析證明書 (CoA)

生物體	Human
組織	Breast, mammary gland
疾病	Invasive ductal carcinoma

生物體	Human
組織	Lung
疾病	Adenocarcinoma (grade III)