OVCAR-4 Cells

650,00 €*

Prix hors taxes plus frais d'expédition

cryovial

Numéro de produit : 305912

Fiche de données de sécurité

Fiche produit

Certificat d'analyse (CoA)

Accord de transfert de matériel

Organisme	Homo sapiens (Human)
Tissus	Metastatic
Maladie	High grade ovarian serous adenocarcinoma
Site métastatique	Ascites
Synonymes	OVCAR 4, NIH:OVCAR-4, NIH:OVCAR4, OVCAR.4, OVCAR4, Ovcar4

L'âge	42Y
Genre	Female
Ethnicité	Caucasian

Citation	OVCAR-4 (Cytion catalog number 305912)
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1627

Profil mutationnel	Mutation: p.Leu130Val, Homozygous

Temps de doublement	34 hours ; 43 hours ; 41.4 hours
Congélation du milieu	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions de stockage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Propriétés "OVCAR-4 Cells"

Site métastatique:	Ascite

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305912-040326	Certificat d'analyse	31. Mar. 2026	305912

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

OVCAR-4 Cells

Informations générales

Caractéristiques

Données réglementaires

Données biomoléculaires

Manipulation

Contrôle de qualité / Profil génétique / HLA

Certificat d'analyse (CoA)

Accord de transfert de matériel