RT-112 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€430.00*

Product number: 300324

Description	This cell line was established by Dr. Carol Rigby, St. Paul?S Hospital, London as described by Benham et al. in 1976 from a human bladder carcinoma.
Organism	Human
Tissue	Bladder
Disease	Carcinoma
Synonyms	RT 112, RT112

Protein expression	p53 positive, Cytokeratine (4),5,(6), 7, 8, 13, 17, 18, 19, Desmoplakin
Isoenzymes	Yes, in nude mice
MSI-status	Stable (MSS)

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	A ratio of 1:4 to 1:8 is recommended
Seeding density	Start culture at 2 to 3 x 10^4 cells/cm^2 and continue with a seeding density of 1 x 10^4 cells/cm^2.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,y CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 12,11 TH01: 7 TPOX: 8,11 vWA: 14,17 D3S1358: 15 D21S11: 27,3 D18S51: 15 Penta E: 12,16 Penta D: 10,11 D8S1179: 13,15 FGA: 23
HLA alleles	A: '26:01:01 B: '27:05:02 C: '01:02:01 DRB1: '01:01:01 DQA1: '01:01:01 DQB1: '05:01:01 DPB1*: '01:01:01 E: '01:01:01

Lot Number	Certificate Type	Date	Catalog Number
300324-040425	Certificate of Analysis	23. May. 2025	300324
300324-070923	Certificate of Analysis	23. May. 2025	300324

General information