NCI-H226 Cells
















Basic details about NCI-H226 cells
Description | The NCI-H226 cell line is derived from a human non-small cell lung carcinoma (NSCLC), specifically squamous cell carcinoma, and is a robust model for studying NSCLC pathogenesis and therapeutic responses. Characterized by its epithelial morphology, NCI-H226 has been utilized extensively in preclinical research focusing on squamous differentiation and apoptosis. This cell line has been pivotal in elucidating the mechanisms of squamous differentiation, particularly the formation of cross-linked envelopes (CLEs) and the role of transglutaminase activity, both of which are markers of terminal differentiation. One key finding associated with NCI-H226 is its response to agents such as suramin, which induces differentiation and apoptosis without necessarily inhibiting cell proliferation. Studies have demonstrated that suramin can stimulate involucrin expression, enhance cytosolic transglutaminase activity, and induce CLE formation in a protein synthesis-independent manner. These effects make NCI-H226 an ideal system for investigating therapeutic agents that exploit cellular differentiation pathways to combat resistant NSCLC. NCI-H226 has also been included in broader cancer research efforts, such as the NCI-60 drug screening program, providing insights into its pharmacological profiles and its utility in high-throughput drug screening. This cell lines genetic and phenotypic stability further solidify its importance in cancer research and therapeutic development. |
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Organism | Human |
Tissue | Lung |
Disease | Pleural epithelioid mesothelioma |
Synonyms | NCI-H226, NCI.H226, NCI H226, H-226, HUT-226, HUT 226, NCIH226 |
Details
Gender | Male |
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Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Specification
Citation | NCI-H226 (Cytion catalog number 305091) |
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Biosafety level | 1 |
Genetic profile of the NCI-H226 cell line
Maintenance techniques
Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality verification of H226 cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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Certificate of Analysis (CoA)
Lot Number | Certificate Type | Date | Catalog Number |
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305091-110724 | Certificate of Analysis | 23. May. 2025 | 305091 |