RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
Quality Control
- Sterile-filtered
Storage and Shelf Life
- Store at +2°C to +8°C, protected from light.
- Once opened, store at 4°C and use within 6–8 weeks.
Shipping Conditions
- Ambient temperature
Maintenance
- Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
- Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
- If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
| Category | Components | Concentration (mg/L) |
| Amino Acids | Glycine | 10.00 |
| L-Alanyl-L-Glutamine | 434.40 | |
| L-Arginine | 200.00 | |
| L-Asparagine H2O | 56.82 | |
| L-Aspartic Acid | 20.00 | |
| L-Cystine 2HCl | 65.20 | |
| L-Glutamic Acid | 20.00 | |
| L-Histidine HCl H2O | 20.27 | |
| L-Hydroxy-L-Proline | 20.00 | |
| L-Isoleucine | 50.00 | |
| L-Leucine | 50.00 | |
| L-Lysine HCl | 40.00 | |
| L-Methionine | 15.00 | |
| L-Phenylalanine | 15.00 | |
| L-Proline | 20.00 | |
| L-Serine | 30.00 | |
| L-Threonine | 20.00 | |
| L-Tryptophan | 5.00 | |
| L-Tyrosine 2Na 2H2O | 28.83 | |
| L-Valine | 20.00 | |
| Vitamins | p-Amino Benzoic Acid | 1.00 |
| D-Biotin | 0.20 | |
| Choline Chloride | 3.00 | |
| D-Calcium Pantothenate | 0.25 | |
| Folic Acid | 1.00 | |
| myo-Inositol | 35.00 | |
| Nicotinamide | 1.00 | |
| Pyridoxine HCl | 1.00 | |
| Riboflavin | 0.20 | |
| Thiamine HCl | 1.00 | |
| Vitamin B12 | 0.005 | |
| Inorganic Salts | Ca(NO3)2 4H2O | 100.00 |
| KCl | 400.00 | |
| MgSO4 7H2O | 100.00 | |
| NaCl | 6000.00 | |
| NaHCO3 | 2000.00 | |
| Na2HPO4 | 800.00 | |
| Other Components | D-Glucose | 2000.00 |
| L-Glutathione Reduced | 1.00 | |
| Phenol Red Sodium Salt | 5.30 |
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