NCI-H1563 Cells












General information
Description | The NCI-H1563 cell line is derived from a human non-small cell lung carcinoma (NSCLC) and is part of the NCI-Navy Medical Oncology Branch collection. This cell line originates from a lung adenocarcinoma, a subtype of NSCLC, highlighting its utility in studying lung cancer pathogenesis and drug responses. It is a model for exploring cellular and molecular mechanisms of NSCLC, which constitutes a significant proportion of lung cancer cases worldwide. NCI-H1563 has been characterized extensively in genomic and proteomic studies, including tyrosine kinase signaling pathways, which are pivotal in lung cancer progression. It has been noted for its phosphotyrosine signaling profile, contributing to understanding activated receptor tyrosine kinases and non-receptor tyrosine kinases in NSCLC. Such pathways are key targets for precision therapies, emphasizing the importance of this cell line in translational cancer research. As part of a larger database of cancer cell lines, NCI-H1563 has also been utilized to analyze genetic mutations, copy number variations, and chromosomal alterations. It contributes to studies aimed at distinguishing driver mutations from passenger mutations in cancer genomics. These features make NCI-H1563 a valuable tool for identifying therapeutic targets, studying resistance mechanisms, and developing personalized treatment strategies for lung cancer. |
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Organism | Human |
Tissue | Lung |
Disease | Lung adenocarcinoma |
Synonyms | NCI-H1563, H-1563, NCIH1563 |
Characteristics
Age | Age unspecified |
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Gender | Male |
Ethnicity | European |
Morphology | Fibroblast-Like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NCI-H1563 (Cytion catalog number 305131) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 9,14
D16S539: 9,13
D5S818: 12,13
D7S820: 7,8
TH01: 6
TPOX: 8,11
vWA: 17,18
D3S1358: 16,17
D21S11: 28,3
D18S51: 13,17
Penta E: 10,13
Penta D: 12,15
D8S1179: 13
FGA: 21,23
D6S1043: 12,13
D2S1338: 16,22
D12S391: 20,23
D19S433: 12,16
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Certificate of Analysis (CoA)
Lot Number | Certificate Type | Date | Catalog Number |
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305131-220923 | Certificate of Analysis | 23. May. 2025 | 305131 |