A3 Cells








General information
Description | A3 cells are human T lymphoblasts derived from the Jurkat cell line obtained from the laboratory of Gerald Crabtree at Stanford University. These cells possess a lymphoblast morphology, grow in suspension and are highly relevant in studying acute T cell leukaemia, 3D cell culture applications, immune system disorder research, and immunology. These cells are derived from the Jurkat cell line, which was treated with Fas Antibody to obtain a low spontaneous resistance rate to Fas-mediated apoptosis. This characteristic makes A3 cells highly valuable for investigating immune system dysregulation and identifying potential therapeutic targets. |
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Organism | Human |
Tissue | Peripheral blood |
Disease | Childhood T acute lymphoblastic leukemia |
Characteristics
Morphology | Lymphoblast |
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Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | A3 (Cytion catalog number 305143) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 8,11
D16S539: 11
D5S818: 9
D7S820: 8,1
TH01: 6,9.3
TPOX: 8,1
vWA: 17,18
D3S1358: 15,17
D21S11: 31.2,33.2
D18S51: 13,20,21
Penta E: 10,12
Penta D: 11
D8S1179: 12,14
FGA: 19,22
D6S1043: 11
D2S1338: 19,23
D12S391: 22,24
D19S433: 13,15.2
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Certificate of Analysis (CoA)
Lot Number | Certificate Type | Date | Catalog Number |
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305143-260724 | Certificate of Analysis | 23. May. 2025 | 305143 |