ZR-75-30 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305389

安全資料表

產品說明書

分析證明書 (CoA)

說明	ZR-75-30 is a human breast cancer cell line derived from a ductal carcinoma. Genomic profiling studies have shown that ZR-75-30 harbors amplification of the ERBB2/HER2 gene, a key driver in a subset of breast cancers. This amplification results in elevated HER2 protein expression, which has been linked to increased proliferation and resistance to certain therapies. Additionally, ZR-75-30 exhibits alterations in the epidermal growth factor receptor (EGFR) signaling pathway, including gains of EGFR-related genes, suggesting that the cell line may be useful in studying HER2-targeted therapies and their resistance mechanisms. Transcriptomic analyses have placed ZR-75-30 within the luminal subtype of breast cancer, supporting its relevance for studying endocrine therapy responses. The cell line has been included in studies evaluating precision medicine approaches, where molecular profiling has helped predict responses to targeted treatments. Given its molecular characteristics, ZR-75-30 is widely used as a preclinical model for evaluating hormone receptor-targeted therapies and HER2 inhibitors, making it a valuable tool in breast cancer research.
生物體	Human
組織	Breast, Mammary gland
疾病	Invasive breast carcinoma of no special type
轉移部位	Ascites
同義詞	ZR75-30, ZR7530

年齡	47 years
性別	Female
族裔	African American
形態學	Epithelial
細胞類型	Epithelial
生長特性	Adherent

引用	ZR-75-30 (Cytion catalog number 305389)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1661

突變譜	Mutation: Gene fusion, APPBP2 + HGNC, PHF20L1, Name(s)=APPBP2-PHF20L1.Gene fusion, BCAS3 + HGNC, HOXB9, Name(s)=BCAS3-HOXB9. Gene fusion, COL14A1 + HGNC, SKAP1, Name(s)=COL14A1-SKAP1. Gene fusion, DDX5 + HGNC, DEPTOR, Name(s)=DDX5-DEPTOR. Gene fusion, BCAS3 + HGNC, ERBB2, Name(s)=ERBB2-BCAS3. Gene fusion, ENPP2 + HGNC, PLEC, Name(s)=PLEC-ENPP2, PLEC1-ENPP2. Gene fusion, PCGF2 + HGNC, TAOK1, Name(s)=TAOK1-PCGF2. Gene fusion, NRIP1 + HGNC, TIAM1, Name(s)=TIAM1-NRIP1. Gene fusion, ARHGAP32 + HGNC, TIMM23, Name(s)=TIMM23-ARHGAP32. Gene fusion, LASP1 + HGNC, TRPS1, Name(s)=TRPS1-LASP1. Gene fusion, CWC25 + HGNC, USP32, Name(s)=USP32-CWC25, USP32-CCDC49. Gene fusion, OPRD1 + HGNC, ZMYM4, Name(s)=ZMYM4-OPRD1. Mutation, BRAF, Simple, p.Ile326Thr (c.977T>C), Heterozygous, CDH1, Simple, p.Glu243Ter (c.727G>T), Homozygous.

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS, 10 µg/ml Insulin
倍增時間	110 hours
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305389-180325	分析證明書	23. May. 2025	305389

ZR-75-30 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)