U-87 MG-RFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305702

安全資料表

產品說明書

分析證明書 (CoA)

說明	U-87 MG-RFP cells are a fluorescently labeled variant of the human glioblastoma astrocytoma cell line U-87 MG, which was originally derived from a malignant glioma tumor of an adult patient. The parental U-87 MG line is widely used as an in vitro model for glioblastoma due to its well-characterized growth properties, tumorigenic potential, and relevance to high-grade astrocytic tumors. These cells exhibit adherent growth with epithelial-like morphology and are commonly employed to study processes such as cell proliferation, invasion, angiogenesis, and response to hypoxic conditions. In U-87 MG-RFP cells, stable expression of red fluorescent protein (RFP) enables real-time visualization of tumor cell behavior in both in vitro and in vivo systems. This modification facilitates applications such as live-cell imaging, tumor tracking in orthotopic xenograft models, and analysis of invasive growth patterns within brain tissue. U-87 MG-RFP cells are particularly valuable for studying glioblastoma progression, tumor–microenvironment interactions, and evaluating therapeutic strategies using fluorescence-based imaging approaches.
生物體	Human
組織	Brain
疾病	Glioblastoma
同義詞	U-87MG, U87 MG, U-87-MG, U87-MG, U-87 MG, U-87, U87, 87 MG, 87MG

年齡	44 years
性別	Male
族裔	Caucasian
形態學	Epithelial-like
生長特性	Adherent

引用	U87MG-RFP (Cytion catalog number 305702)
生物安全等級	1
NCBI_TaxID	9606
轉基因狀態	GMO-S1: This human glioblastoma RFP-tagged cell line (U87MG-RFP) contains a lentiviral construct encoding red fluorescent protein from Aequorea victoria, enabling stable red fluorescence labeling. The modification is stably present. This classification applies only within Germany and may differ elsewhere.

蛋白質表達	RFP
同工酶	Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B
致瘤性	Yes, in nude mice inoculated subcutaneously with 107 cells

培養基	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
播種密度	1.5 to 2 x 10⁴ cells/cm²
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
305702-170326	分析證明書	13. Apr. 2026	305702

U-87 MG-RFP Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)