SW620-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305708

安全資料表

產品說明書

分析證明書 (CoA)

說明	SW620-GFP cells are a fluorescently labeled variant of the human colorectal adenocarcinoma cell line SW620, which was originally derived from a metastatic lesion in a lymph node of an adult patient. These cells have been engineered to stably express green fluorescent protein (GFP), enabling direct visualization of cellular morphology, proliferation, and migratory behavior in real time using fluorescence-based imaging techniques. The parental SW620 line is characterized by its metastatic origin and exhibits features associated with advanced colorectal cancer, including enhanced motility, invasive capacity, and altered cell adhesion properties. The expression of GFP in SW620-GFP cells facilitates applications such as live-cell tracking, invasion assays, and in vivo imaging in xenograft models, where tumor growth and dissemination can be monitored non-invasively. These cells retain key molecular characteristics of the parental line, including mutations commonly associated with colorectal cancer progression and dysregulation of signaling pathways such as Wnt/β-catenin and MAPK. As a result, SW620-GFP cells are a valuable tool for studying mechanisms of metastasis, tumor microenvironment interactions, and the evaluation of anti-cancer therapeutics targeting advanced-stage colorectal carcinoma.
生物體	Human
組織	Metastatic
疾病	Colon adenocarcinoma
轉移部位	Lymph node
同義詞	SW620, SW 620, SW.620

年齡	51 years
性別	Male
族裔	Caucasian
形態學	Epithelial-like
生長特性	Suspension

引用	SW620-GFP (Cytion catalog number 305708)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_A9MJ
轉基因狀態	GMO-S1: This SW-620 metastatic colorectal line contains a GFP construct for visualization of metastatic behavior. This classification applies only within Germany and may differ elsewhere.

蛋白質表達	GFP
致瘤性	Yes, in athymic nude mice
突變譜	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

培養基	DMEM
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

屬性 "SW620-GFP Cells"

轉移部位:	Lymph node

批次編號	證書類型	日期	型號
305708-230326	分析證明書	23. Apr. 2026	305708

SW620-GFP Cells

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品質控制與分子分析

分析證明書 (CoA)