SW527 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300640

安全資料表

產品說明書

分析證明書 (CoA)

說明	The SW527 cell line is a human breast carcinoma cell line derived from a Caucasian adult patient. It was established in the early 1970s and has been included in foundational studies characterizing tumorigenicity in immunodeficient models. In one such study, SW527 successfully formed tumors in nude mice following subcutaneous inoculation of 6 × 10⁶ cells, supporting its malignant origin. Histopathological analysis of the resulting tumors showed features consistent with the original human carcinoma, confirming its relevance as a breast cancer model. SW527 has been authenticated as a tumor-derived line of G6PD type B, a classification that helps exclude contamination with HeLa cells, which is a critical concern in historical cell line collections. Despite this, comprehensive molecular or immunological profiling of SW527 appears limited in recent large-scale datasets. Overall, SW527 remains a validated breast carcinoma model, primarily supported by in vivo tumorigenicity data. Additional molecular profiling would be beneficial to broaden its utility in mechanistic or drug discovery research.
生物體	Human
組織	Breast; Mammary gland
疾病	Breast adenocarcinoma
同義詞	SW-527, SW 527

年齡	70 years
性別	Female
族裔	Caucasian
形態學	Epithelial
細胞類型	Epithelial
生長特性	Adherent

引用	SW527 (Cytion catalog number 300640)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_3799

突變譜	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
300640-120925	分析證明書	05. Dec. 2025	300640

SW527 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)