SU-DHL-8 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305877

安全資料表

產品說明書

分析證明書 (CoA)

說明	SU-DHL-8 is a human diffuse large B-cell lymphoma (DLBCL) cell line derived from an adult patient. It represents the activated B-cell-like (ABC) subtype of DLBCL, which is characterized by constitutive activation of the NF-κB signaling pathway and typically exhibits poorer prognosis compared to the germinal center B-cell-like (GCB) subtype. Morphologically, the SU-DHL-8 cells grow as large, loosely adherent aggregates in suspension, consistent with B-cell lymphoma phenotypes. Molecular characterization reveals that SU-DHL-8 harbors mutations commonly associated with ABC-DLBCL, including alterations affecting the BCR and NF-κB signaling pathways. Genomic profiling through next-generation sequencing and expression analysis has identified elevated activity in pathways such as JAK/STAT and BCL2-associated anti-apoptotic signaling. The cell line is also part of several large-scale pharmacogenomic studies and cancer model repositories, where it has been used to explore drug sensitivities, particularly to kinase inhibitors and proteasome-targeting agents. These features make SU-DHL-8 a representative and valuable model for investigating the molecular pathogenesis and therapeutic vulnerabilities of ABC-type DLBCL.
生物體	Human
組織	Pleural effusion
疾病	Diffuse large B-cell lymphoma germinal center B-cell type
同義詞	SUDHL8, SUDHL-8, SuDHL 8, Stanford University-Diffuse Histiocytic Lymphoma-8, DHL-8, DHL8

年齡	59 years
性別	Male
族裔	Caucasian
形態學	Lymphoblast-like
細胞類型	B lymphocyte
生長特性	Suspension, single cells and small clusters

引用	SU-DHL-8 (Cytion catalog number 305877)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_2207

抗原表達	Ig+; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa-
突變譜	Mutation: KMT2D, Simple, p.Pro648Thrfs*2 (c.1940dupC) (c.1940_1941insC), Heterozygous (Cosmic-CLP=1331038), TP53, Simple, p.Tyr234Asn (c.700T>A), Heterozygous (Cosmic-CLP=1331038), TP53, Simple, p.Arg249Gly (c.745A>G), Heterozygous (Cosmic-CLP=1331038)

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	none
倍增時間	~48-72 hours
播種密度	0.3-0.5 x 10⁶ cells/ml
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305877-301025	分析證明書	05. Dec. 2025	305877

SU-DHL-8 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)

生物體	人類
組織	胸腔積液
疾病	未分化大細胞淋巴瘤，ALK 陽性