RWPE-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$450.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305217

安全資料表

產品說明書

分析證明書 (CoA)

說明	The RWPE-1 cell line, derived from the prostate epithelium of a 54-year-old Caucasian male with no evidence of prostate cancer, is a valuable resource in biomedical research, particularly for studies on prostate biology and cancer. These epithelial cells, characterized by their adherence growth properties and typical epithelial morphology, were immortalized using a replication-deficient retrovirus that carries the E7 gene from human papillomavirus 18 (HPV-18), which inactivates the retinoblastoma protein and promotes cellular immortalization. RWPE-1 cells, originating from a normal human prostate, are utilized in prostate cancer research, though their androgen receptor expression is relatively modest, especially when compared to tumorigenic prostate cancer-derived cell lines. The epithelial cell line RWPE-1 expresses cytokeratins 8 and 18, which confirm their epithelial lineage. While RWPE-1 cells do express tumor suppressors such as p53 and pRB, reflecting their non-tumorigenic nature, the expression of prostate-specific markers like Kallikrein 3 (KLK3) or PSA is generally low or absent under standard culture conditions. In 3D cultures, such as those formed in Matrigel, human cells RWPE-1 can organize into acinar structures reminiscent of normal prostate architecture. When it comes to the secretion of PSA (Prostate-Specific Antigen) in response to androgen stimulation, RWPE-1 cells show a less pronounced reaction compared to prostate cancer cell lines. Therefore, while RWPE-1 cells offer a valuable model for understanding the baseline properties of normal prostate epithelial cells. RWPE-1's non-tumorigenic nature serves as a model for studying the transition to tumorigenic transformation and the dynamics of cancer cells, including metastatic prostate cancer cells and prostate carcinogenesis. The inclusion of factors like EGF and growth hormone in culture conditions can further elucidate the pathways involved in prostatic hyperplasia and the progression towards prostate cancer. In summary, RWPE-1 cells facilitate a comprehensive understanding of prostate cancer, from its initiation in prostatic cell lines to its manifestation in prostate cancer patients.
生物體	Human
組織	Prostate
同義詞	RWPE1

年齡	54 years
性別	Male
族裔	Caucasian
形態學	Epithelial
細胞類型	Epithelial cell of prostate
生長特性	Adherent

引用	RWPE-1 (Cytion catalog number 305217)
生物安全等級	RWPE-1 is classified as Biosafety Level 1 or 2 (BSL-1/2) in Germany, depending on the type of work conducted. The cell line originates from human prostate epithelial cells transfected with a single copy of HPV-18 and is negative for Hepatitis B, Hepatitis C, and HIV. Viral particle release is unlikely, as HPV-18 requires differentiated epithelial cells for replication, and a single genome copy does not typically lead to particle formation. Such release is only theoretically possible in 3D cultures (e.g., organotypic or raft cultures) but is excluded in monolayer cultures. Due to the presence of the full HPV-18 genome, RWPE-1 is categorized as a Risk Group 2 organism for genetic engineering purposes.
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_3791

核型分析	RWPE-1 cells have a diploid chromosomal ploidy, and show chromosomal variations such as 45, X,-Y, and 51, XY.

培養基	K-SFM (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance)
營養補充品	Supplement the medium with 0.05 mg/mL BPE, 5 ng/mL EGF. The medium should not be entirely filtered. Add BPE and EGF to 10 mL, and after sterile filtering, incorporate this mixture into the medium.
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305217-310124	分析證明書	23. May. 2025	305217
305217-021025	分析證明書	05. Dec. 2025	305217

RWPE-1 Cells

Essential facts about RWPE-1 cells

Details

Documentation about RWPE-1 epithelial cells

Genotype

Handling

Quality verification of RWPE-1 prostate cell lines

分析證明書 (CoA)