RAW 264.7-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305699

安全資料表

產品說明書

分析證明書 (CoA)

說明	RAW 264.7-GFP cells are a genetically modified derivative of the murine RAW 264.7 macrophage-like cell line, which originates from an adult mouse tumor of monocyte/macrophage lineage. These cells have been engineered to express green fluorescent protein (GFP), enabling real-time visualization of cellular processes such as morphology, migration, and phagocytic activity under fluorescence microscopy. The parental RAW 264.7 line is widely used as a model for innate immune responses, as it retains many functional characteristics of activated macrophages, including the ability to produce cytokines, nitric oxide, and reactive oxygen species upon stimulation. The incorporation of GFP allows for dynamic, non-invasive monitoring of macrophage behavior in vitro, making RAW 264.7-GFP cells particularly valuable for live-cell imaging studies and high-content screening applications. These cells respond robustly to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and interferon-gamma, leading to activation of signaling pathways including NF-κB and MAPK. This makes them a useful model for investigating inflammatory signaling, host-pathogen interactions, and the effects of pharmacological agents on macrophage activation. However, as with the parental line, researchers should consider that RAW 264.7-derived models may exhibit altered regulatory pathways compared to primary macrophages.
生物體	Mouse
組織	Ascites
疾病	Mouse leukemia
同義詞	GFP/RAW264.7

年齡	Adult
性別	Male
生長特性	Adherent

引用	RAW 264.7-GFP (Cytion catalog number 305699)
生物安全等級	2
NCBI_TaxID	10090
Cellosaurus 編號	CVCL_D7C8

蛋白質表達	GFP

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
播種密度	2 to 5 x 10⁴ cells/cm²
流體更新	3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
305699-260226	分析證明書	26. Mar. 2026	305699

RAW 264.7-GFP Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)