NCI-H929 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305236

安全資料表

產品說明書

分析證明書 (CoA)

說明	The NCI-H929 cell line is a human myeloma cell line derived from the bone marrow of a patient with multiple myeloma, a type of cancer that forms in plasma cells. These cells are particularly useful in cancer research due to their ability to produce large amounts of immunoglobulin, making them a prime model for studying the biology of multiple myeloma and the mechanisms of immunoglobulin production. The NCI-H929 cells grow as a suspension culture and have a doubling time of approximately 40 hours, making them relatively easy to propagate in laboratory conditions. Genetically, NCI-H929 cells exhibit several chromosomal abnormalities commonly associated with multiple myeloma, including translocations and amplifications. These genetic features make them an invaluable resource for studying the genetic underpinnings of myeloma and testing potential therapeutic interventions. Researchers often utilize NCI-H929 cells in drug screening assays to evaluate the efficacy of new anti-myeloma compounds and to understand drug resistance mechanisms. Their consistent and reproducible behavior under various experimental conditions further enhances their utility in preclinical studies.
生物體	Human
組織	Bone marrow
疾病	Multiple myeloma
轉移部位	Pleural effusion
同義詞	NCI H929, NCIH929, H929, H-929

年齡	62 years
性別	Female
族裔	European
形態學	Lymphoblast
細胞類型	B lymphocyte
生長特性	Suspension

引用	NCI-H929 (Cytion catalog number 305236)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1600

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
傳代培養	Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.