NCI-H2122 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305600

安全資料表

產品說明書

分析證明書 (CoA)

說明	The NCI-H2122 cell line is a human non-small cell lung cancer (NSCLC) model derived from an adenocarcinoma patient. It is notable for its harboring of a KRAS G12C mutation, a hallmark of NSCLC that leads to constitutive activation of the MAPK signaling pathway. This cell line is extensively used in studies focusing on therapeutic interventions targeting KRAS G12C and associated downstream pathways, particularly those involving MEK and ERK inhibitors. Research utilizing NCI-H2122 has highlighted its role in understanding drug resistance mechanisms and in optimizing combination therapies. Preclinical studies using the NCI-H2122 cell line have demonstrated its utility in exploring resistance to MAPK pathway inhibitors. For instance, CRISPR screening approaches have identified MAPK7 (ERK5) as a critical mediator of pathway reactivation following MEK inhibition, suggesting potential combination strategies using MEK inhibitors like cobimetinib and MAPK7 inhibitors. The line also serves as a model for evaluating the efficacy of small molecule inhibitors, including those targeting PI3K and BRAF, which are relevant in combination with KRAS-specific treatments. NCI-H2122 is also employed in investigating metabolic vulnerabilities in NSCLC. Studies have implicated serine biosynthesis and the folate cycle as metabolic pathways contributing to resistance against targeted therapies, such as BRAF inhibitors. Metabolic modulators like methotrexate and serine-deprivation strategies have been tested on this cell line, providing insights into overcoming drug resistance and identifying new metabolic targets for therapeutic exploitation.
生物體	Human
組織	Lung
疾病	Adenocarcinoma
轉移部位	Pleural effusion
同義詞	H2122, H-2122, NCIH2122

年齡	46 years
性別	Female
族裔	Caucasian
形態學	Epithelial-like, Lymphoblast-like
生長特性	Adherent

引用	NCI-H2122 (Cytion catalog number 305600)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1531

突變譜	Mutation: KRAS, p.Gly12Cys (c.34G>T), homozygous; Mutation: TP53, p.Gln16Leu (c.47A>T), heterozygous; Mutation: TP53, p.Cys176Phe (c.527G>T), heterozygous

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.