NCI-H2087 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305824

安全資料表

產品說明書

分析證明書 (CoA)

說明	NCI-H2087 is a human non-small cell lung carcinoma (NSCLC) cell line derived from the metastatic site (specifically, a lymph node) of an adult patient with lung adenocarcinoma. This cell line is epithelial in morphology and is commonly utilized in studies investigating lung cancer pathogenesis, therapeutic responses, and molecular profiling of metastatic adenocarcinomas. It exhibits characteristics consistent with its origin, including expression of epithelial markers and various genetic alterations typical of lung adenocarcinomas. Genetically, NCI-H2087 is known to harbor mutations relevant to oncogenesis and therapy resistance in NSCLC. Most notably, it contains a KRAS mutation, which is associated with constitutive activation of downstream signaling pathways such as MAPK and PI3K-AKT, leading to enhanced cell proliferation and survival. The presence of this mutation makes NCI-H2087 a valuable model for studying KRAS-driven tumorigenesis and for evaluating targeted inhibitors that disrupt KRAS signaling. Additionally, the cell line is p53 mutant, which can contribute to impaired apoptosis and genomic instability, further supporting its utility in preclinical cancer biology and drug screening research.
生物體	Human
組織	Lymph node
疾病	Lung adenocarcinoma
同義詞	H2087, H-2087, NCIH2087

年齡	69 years
性別	Male
族裔	Caucasian
形態學	Epithelial-like and/or rounded
生長特性	Adherent

引用	NCI-H2087 (Cytion catalog number 305824)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1524

突變譜	Mutation: ATM, Simple, p.Glu848Gln (c.2542G>C), Heterozygous, BRAF, Simple, p.Leu597Val (c.1789C>G), Heterozygous, MYC, Simple, p.Glu54Lys (c.160G>A), Heterozygous, NRAS, Simple, p.Gln61Lys (c.181C>A), Heterozygous, TP53, Simple, p.Val157Phe (c.469G>T), Homozygous

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	51 hours
解離試劑	Accutase
播種密度	4 x 10⁴ cells/cm²
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
305824-101025	分析證明書	05. Dec. 2025	305824

NCI-H2087 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)