MML-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300288

安全資料表

產品說明書

說明	The MML-1 cell line is a melanoma cell line derived from malignant melanoma. This cell line is used primarily to study melanoma biology, particularly the role of extracellular vesicles (EVs) in cell-to-cell communication and tumor progression. MML-1 cells release various subtypes of EVs, including exosomes, microvesicles, and apoptotic bodies, each carrying distinct RNA cargos, such as microRNAs (miRNAs) and other non-coding RNAs. Studies using MML-1 cells have demonstrated that exosomes released from these cells contain specific miRNAs like miR-214-3p, miR-199a-3p, and miR-155-5p, which are closely associated with melanoma progression and metastasis. These miRNAs are enriched in exosomes compared to other EV types and have been linked to important melanoma-related pathways, such as the regulation of the MAPK signaling pathway and tumor microenvironment interactions. Interestingly, comparisons of miRNA profiles from MML-1-derived exosomes with clinical samples of melanoma show a significant overlap, indicating the clinical relevance of this cell model in understanding melanoma progression. In addition to miRNAs, MML-1 cells also release other non-coding RNAs such as small nucleolar RNAs (snoRNAs) and mitochondrial-associated transfer RNAs (mt-RNAs), which are differentially distributed among the EV subtypes. These findings highlight the MML-1 cell line's utility in studying the molecular mechanisms of melanoma, particularly how tumor cells communicate via EVs and influence their microenvironment.
生物體	Human
組織	Skin
疾病	Melanoma
同義詞	MML1

年齡	Unspecified
性別	Unspecified
形態學	Epithelial-like
生長特性	Adherent

引用	MML-1 (Cytion catalog number 300288)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_6004

蛋白質表達	P53 positive
致瘤性	Yes, in nude mice
逆轉錄酶	Negative
突變譜	V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot).

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm²
流體更新	2 to 3 times per week
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

MML-1 Cells

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