MHH-ES1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300136

安全資料表

產品說明書

說明	The MHH-ES1 cell line is derived from a patient with Ewing sarcoma, a highly aggressive bone and soft tissue cancer predominantly affecting children and young adults. This cell line is a valuable model for studying the molecular mechanisms underlying Ewing sarcoma, particularly the role of the EWSR1-FLI1 fusion gene, which is characteristic of this cancer type. The fusion gene results from a translocation between chromosomes 11 and 22, leading to the production of an oncogenic transcription factor that drives tumorigenesis. MHH-ES1, like other Ewing sarcoma cell lines, is utilized to investigate the pathways influenced by EWSR1-FLI1, including alterations in cell proliferation, differentiation, and apoptosis. Researchers use the MHH-ES1 cell line to evaluate the efficacy of various therapeutic agents targeting pathways critical for Ewing sarcoma survival and proliferation. For example, it is instrumental in testing small molecule inhibitors, RNA interference, and CRISPR-Cas9 gene editing techniques aimed at disrupting the EWSR1-FLI1 fusion gene or its downstream effectors. Additionally, MHH-ES1 serves as a model to study resistance mechanisms to conventional chemotherapy and to identify novel biomarkers for early diagnosis and treatment response monitoring in Ewing sarcoma patients.
生物體	Human
組織	Bone
疾病	Ewing's Sarcoma
轉移部位	Ascites
同義詞	MHH-ES-1, MHHES1

年齡	12 years
性別	Male
族裔	Turkish
形態學	Small round cells
生長特性	Adherent, clusters

引用	MHH-ES1 (Cytion catalog number 300136)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1411

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 to 2 x 10⁴ cells/cm²
流體更新	Every 3 to 5 days
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

MHH-ES1 Cells

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