LP-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300321

安全資料表

產品說明書

分析證明書 (CoA)

說明	The LP-1 cell line is a well-established human multiple myeloma cell line derived from a patient with multiple myeloma. It is characterized by its t(4;14)(p16;q32) translocation, which results in the dysregulated expression of fibroblast growth factor receptor 3 (FGFR3). This genetic aberration is a hallmark of a subset of multiple myeloma cases and is associated with the pathogenesis and progression of the disease. LP-1 cells express a functional FGFR3, which, when activated, can engage the MAP kinase signaling pathway, promoting cell proliferation and survival. Notably, LP-1 carries a non-activating F384L mutation in the FGFR3 gene, distinguishing it from other myeloma cell lines with activating mutations of FGFR3. LP-1 cells are useful for studying the role of FGFR3 in multiple myeloma, particularly in the context of non-activating mutations. Research has shown that in multiple myeloma, FGFR3 mutations and other common oncogenic mutations, such as those in the Ras family, are typically mutually exclusive, suggesting that these mutations may contribute to tumorigenesis through similar or overlapping pathways. This makes LP-1 an invaluable model for exploring the molecular mechanisms underlying multiple myeloma and for testing targeted therapies aimed at the FGFR3 pathway. In addition to its relevance in FGFR3-related studies, LP-1 is also significant in research focused on the broader aspects of myeloma biology, including the role of cytokines like interleukin-6 (IL-6) in cell survival and proliferation. This cell line has been instrumental in studies that investigate the interactions between myeloma cells and their bone marrow microenvironment, as well as in the development of novel therapeutic strategies aimed at disrupting these interactions to control disease progression.
生物體	Human
組織	Peripheral blood
疾病	Multiple myeloma
應用	Model to study the process of B lymphocyte maturation.
同義詞	LP1

年齡	56 years
性別	Female
形態學	Elongated single cells
生長特性	Suspension

引用	LP-1 (Cytion catalog number 300321)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0012

產品	IgG lambda
核型分析	Chromosome modal numer 73, distribution from 60 to 79 chromosomes

培養基	IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a)
營養補充品	Supplement the medium with 20% heat inactivated FBS
傳代培養	It is recommended to seed the cells into a 24 well plate and cultivate for one week after thawing. Exchange the medium by dilution. Later on, the cells can be cultivated in regular cell culture flasks. Maintain culture between 0.5 to 1 x10⁶ cells/ml. Incubate at 5% CO₂, 37 degree Celsius.
播種密度	7 x 10⁵ cells/well of a 24 to well to plate.
解凍後的恢復	Viability may be low after thawing.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300321-070125	分析證明書	23. May. 2025	300321
300321-240325	分析證明書	23. May. 2025	300321

LP-1 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)