L-929-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305956

安全資料表

產品說明書

說明	L-929-GFP cells are a fluorescently labeled derivative of the murine L-929 fibroblast cell line, which was originally established from subcutaneous connective tissue of an adult mouse. The parental L-929 line is one of the most extensively used mouse fibroblast models in biomedical research and is characterized by its adherent growth, spindle-shaped morphology, and robust proliferative capacity. L-929 cells are widely utilized in studies of cytotoxicity, inflammation, extracellular matrix biology, and host–pathogen interactions, and they are also commonly employed for the production and bioassay of cytokines such as tumor necrosis factor-α (TNF-α). The stable expression of green fluorescent protein (GFP) in L-929-GFP cells enables direct visualization and quantitative tracking of fibroblast behavior in real time. These cells are particularly useful for fluorescence-based applications including migration assays, co-culture experiments, tissue engineering studies, and live-cell imaging. L-929-GFP cells retain the core biological characteristics of the parental fibroblast line while providing enhanced utility for monitoring cell localization, proliferation, and interactions within complex cellular environments. Consequently, they serve as a versatile model for investigating stromal cell dynamics, wound healing processes, biomaterial compatibility, and immune-mediated cytotoxic responses.
生物體	Mouse
組織	Connective tissue
同義詞	L929/GL50

年齡	100 days
性別	Male
細胞類型	Fibroblast
生長特性	Adherent

引用	L929-GFP (Cytion catalog number 305956)
生物安全等級	1
NCBI_TaxID	10090
Cellosaurus 編號	CVCL_E2Z7

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 to 3 x 10⁴ cells/cm²
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

L-929-GFP Cells

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