HK-CRISPR-Nup188-mEGFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300657

安全資料表

產品說明書

分析證明書 (CoA)

說明	HK-CRISPR-Nup188-mEGFP is a genetically modified Hela Kyoto cell line. It uses CRISPR/Cas9 technology to integrate mEGFP (monomeric Enhanced Green Fluorescent Protein) at the Nup188 locus. Nup188 is a key nucleoporin protein involved in the nuclear pore complex, essential for molecule transport between the nucleus and cytoplasm. The mEGFP tagging allows real-time visualization of Nup188, aiding in the study of nuclear pore complex dynamics and function. This cell line is particularly useful for researchers focusing on nuclear transport and nuclear pore complex biology. Its fluorescent tagging enables observation of Nup188 under various conditions, including drug treatments and genetic modifications. Applications include high-content screening, live-cell imaging, and other fluorescence-based techniques, providing valuable insights into nucleocytoplasmic transport and nuclear envelope integrity.
生物體	Human
組織	Endocervix
疾病	Adenocarcinoma
轉移部位	Not applicable (HeLa Kyoto derivative; primary tumor site is endocervix)
應用	Nuclear pore complex (NPC) biology; nucleocytoplasmic transport; Nup188 dynamics and function; live-cell fluorescence imaging of nuclear envelope; high-content screening; CRISPR knock-in validation studies; nuclear transport inhibitor evaluation

年齡	30 years
性別	Female
族裔	African American
形態學	Epithelial-like cells with mosaic stone shape
細胞類型	Epithelial cells
生長特性	Adherent

引用	HK-CRISPR-Nup188-mEGFP (Cytion catalog number 300657)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	Not assigned (HK-CRISPR-Nup188-mEGFP is a CRISPR-modified HeLa Kyoto derivative; parental HeLa Kyoto CVCL_1922)
存款人	The Ellenberg Lab (EMBL)
轉基因狀態	GMO-S1: This HeLa Kyoto line contains a CRISPR knock-in of mEGFP at the Nup188 locus, enabling visualization of nuclear pore scaffold dynamics. This classification applies only within Germany and may differ elsewhere.

蛋白質表達	Nup188, mEGFP-tag

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300657-171123	分析證明書	23. May. 2025	300657

HK-CRISPR-Nup188-mEGFP Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)