HEK293-Rpn11-HTBH Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305719

安全資料表

產品說明書

分析證明書 (CoA)

說明	Stable HEK293 Cells-Rpn11-HTBH is a stably transfected derivative of the HEK293 (Human Embryonic Kidney 293) cell line, engineered to express a tagged version of Rpn11 (also known as PSMD14 or POH1), the deubiquitinase subunit of the 26S proteasome lid complex. Rpn11 is a Zn²⁺-dependent JAMM-domain deubiquitinase that removes ubiquitin chains from proteasome-bound substrates during proteasomal degradation. The HTBH tag (hexahistidine-TEV-biotin acceptor peptide-hexahistidine) enables affinity purification of Rpn11-containing complexes under native conditions, making this line particularly suited for proteasome complex purification and interactome studies. This cell line is applicable in studies of 26S proteasome biology, ubiquitin-proteasome pathway (UPS) regulation, Rpn11/PSMD14 function in protein quality control, proteasome assembly and dynamics, and proteasome inhibitor mechanism of action. It is also used for affinity purification of native proteasome complexes and as a model for studying deubiquitinase biology in the context of the proteasome. The HTBH tagging system enables highly stringent purification of biotinylated complexes using streptavidin-based pulldowns. Stable HEK293 Cells-Rpn11-HTBH are maintained as an adherent culture in DMEM supplemented with 10% FBS and the appropriate selection antibiotic to maintain transgene expression at 37°C in a humidified 5% CO₂ atmosphere. Cells are subcultured with Accutase at 80–90% confluency (split ratio 1:5 to 1:10). Medium renewed every 2–3 days.
生物體	Human
組織	Kidney
疾病	Transformed/immortalized fetal kidney (HEK293 background; Rpn11-HTBH transgene)
應用	26S proteasome biology; Rpn11/PSMD14 function; ubiquitin-proteasome pathway; proteasome complex purification; deubiquitinase biology; HTBH-tag affinity purification; proteasome interactome studies

形態學	Epithelial-like
細胞類型	Epithelial cells
生長特性	Adherent

引用	Stable HEK293 Cells-Rpn11-HTBH (Cytion catalog number 305719)
生物安全等級	1
NCBI_TaxID	9606
轉基因狀態	GMO-S1: This HEK293 derivative contains a stably integrated Rpn11-HTBH expression cassette (hexahistidine-TEV-biotin acceptor peptide-hexahistidine tagged Rpn11/PSMD14). This classification applies only within Germany and may differ elsewhere.

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Most cells detach in PBS, if necessary add Accutase 5 min RT
倍增時間	approx. 24 to 36 hours
傳代培養	Remove medium, wash with PBS without calcium and magnesium, cover with Accutase, incubate 8–10 min at RT, resuspend in medium, centrifuge 300×g 3 min, discard supernatant, reseed in fresh medium.
拆股比例	1 to 10
播種密度	2 to 4 × 10⁴ cells/cm²
流體更新	Every 2 to 3 days
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
305719-040526	分析證明書	24. Jun. 2026	305719

HEK293-Rpn11-HTBH Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)