GP2D Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305778

安全資料表

產品說明書

分析證明書 (CoA)

說明	GP2d is a human colorectal adenocarcinoma cell line derived from a poorly differentiated colon tumor. It was established alongside a sister line, GPSd, from the same adenocarcinoma specimen. While both lines share similar genetic alterations consistent with common patterns seen in colorectal cancer, including an inverted duplication involving chromosome 10q11-q21, they differ markedly in their phenotypic characteristics and cellular behavior. Notably, no translocations involving the ret proto-oncogene-mapped to this chromosomal region-were detected by Southern blot analysis, suggesting the duplication did not disrupt this gene directly. GP2d cells exhibit a cohesive, spreading growth pattern from the edges of microcolonies to form a confluent epithelial monolayer. This morphology is accompanied by distinct expression patterns of adhesion molecules such as α2-integrin, desmoplakin, and E-cadherin, all of which play roles in maintaining epithelial integrity. Functionally, GP2d cells respond robustly to epidermal growth factor (EGF), transforming growth factor-alpha (TGFα), and insulin, as demonstrated by increased cell proliferation in response to these ligands. Interestingly, both GP2d and GPSd express comparable numbers of EGF receptors, but differ in their expression of EGF receptor ligands. GP2d cells have abundant amphiregulin mRNA, whereas GPSd predominantly expresses TGFα mRNA with little to no amphiregulin, correlating with the differing biological responses observed. These features make GP2d a valuable model for studying the regulation of growth factor signaling and cell adhesion in colorectal cancer. Its responsiveness to EGF pathway stimuli and distinct epithelial morphology highlight its utility in investigating tumor cell differentiation and proliferation. Furthermore, the shared origin with GPSd allows for comparative studies of clonal variation within tumors, particularly in the context of ligand-receptor dynamics and epithelial-to-mesenchymal transition (EMT) responses.
生物體	Human
組織	Colon
疾病	Adenocarcinoma
同義詞	Gp2d, Gp2D, GP2D

年齡	71 years
性別	Female
族裔	Caucasian
生長特性	Adherent

引用	GP2D (Cytion catalog number 305778)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_2450

突變譜	Mutation: KRAS, Simple, p.Gly12Asp (c.35G>A), Heterozygous, TP53

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305778-200426	分析證明書	15. May. 2026	305778

GP2D Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)