GH3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300383

安全資料表

產品說明書

分析證明書 (CoA)

說明	The GH3 cell line, originating from a rat pituitary tumor, is a critical resource in the study of pituitary functions, particularly regarding the secretion of prolactin and growth hormone. These cells possess characteristics of both somatotropic and lactotropic cells, enabling detailed investigations into pituitary hormones and their regulatory mechanisms. The cell line is extensively utilized to understand the effects of hormonal treatments and genetic modifications on the secretion of these hormones. GH3 cells respond significantly to thyroid-stimulating hormones, making them a valuable model for assays that measure the impact of various compounds on pituitary gland activities. Research employing GH3 cells often delves into how these cells react to different hormonal stimuli. For example, hydrocortisone is known to promote growth hormone production while inhibiting prolactin output in these cells, making GH3 a preferred model for exploring hormonal balance and the endocrine system’s response to stress and other physiological factors. Such studies are pivotal in advancing our understanding of pituitary gland disorders and crafting therapies for conditions like growth deficiencies and hyperprolactinemia. Moreover, GH3 cells are instrumental in pharmacological testing and biotechnological applications aimed at developing treatments for pituitary-related disorders. Their ability to produce more growth hormone compared to GH1 cells, along with prolactin, allows researchers to examine the regulation and effects of these hormones under various conditions. This unique profile is essential for understanding the complex interactions within the endocrine system and for the development of targeted therapeutic interventions.
生物體	Rat
組織	Brain, Pituitary gland
疾病	Neoplasm
同義詞	GH 3

品種／亞種	Wistar Furth
年齡	7 months
性別	Female
形態學	Epithelial-like
生長特性	Adherent, clusters in suspension

引用	GH3 (Cytion catalog number 300383)
生物安全等級	1
NCBI_TaxID	10116
Cellosaurus 編號	CVCL_0273

產品	Growth hormone, prolactin

培養基	Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a)
營養補充品	Supplement the medium with 15% horse serum, 2.5% heat-inactivated FBS
解離試劑	Accutase
傳代培養	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300383-180225	分析證明書	21. Jul. 2025	300383
300383-200223	分析證明書	26. May. 2025	300383
300383-200223SF	分析證明書	23. May. 2025	300383

GH3 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)