EB1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300403

安全資料表

產品說明書

分析證明書 (CoA)

說明	The EB1 cell line is a human-derived cell line established from biopsy fragments and cell clumps of Burkitt lymphoma. This line was originally cultivated in Eagle's basal medium supplemented with 10% human serum. The unique growth conditions facilitated the development of cells that predominantly grew as free-floating single individuals or doublets. The EB1 cells exhibit a characteristic doubling time of approximately 48 hours, highlighting their rapid proliferation rate, which is a hallmark feature of lymphoblasts. Morphologically, the EB1 cells display uniform altered lymphoblast characteristics, indicating their derivation from lymphoid tissue. The cell line has been utilized extensively in the study of Burkitt's lymphoma, providing insights into the pathology of lymphoid malignancies. It serves as a valuable model for researching the biological behavior of lymphoid cells under various experimental conditions, aiding in the exploration of therapeutic targets and the understanding of lymphoma progression.
生物體	Human
組織	Blood
疾病	Burkitt lymphoma
同義詞	EB-1, Epstein-Barr-1

年齡	9 years
性別	Female
族裔	African
形態學	Polymorph cells, big nuclei, formation of microvilli
細胞類型	B lymphocyte
生長特性	Suspension

引用	EB1 (Cytion catalog number 300403)
生物安全等級	2
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_2027

同工酶	PGM1, ESD1, GLO-1, G6PD, B
病毒	Contains Herpesvirus
核型分析	Chromosome Frequency Distribution 30 cells: 2n = 46. The cell line is aneuploid human female, with chromosome counts in the near dipoidrange. Normal chromosomes N8, N11 and N14 are monosomic, with the remainder of autosomes usually being paired. The x chromosome most often is trisomic. Four marker chromosomes are found. Two of these (markers M1 and M3) involve the reciprocal translocation between chromosomes N8 and N14 associated with most Burkitt's lymphoma cell lines.

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% heat-inactivated FBS
倍增時間	48 hours
傳代培養	The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
播種密度	0.1 x 10⁶ cells/ml
流體更新	2 to 3 times per week
解凍後的恢復	After thawing, allow the cells to recover from the freezing process for at least 24 hours
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300403-213	分析證明書	23. May. 2025	300403
300403-240225	分析證明書	23. May. 2025	300403

EB1 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)