DAN-G Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300162

安全資料表

產品說明書

分析證明書 (CoA)

說明	The DAN-G cell line is derived from a human pancreatic carcinoma. It is extensively utilized in research focused on pancreatic cancer, particularly in studies pertaining to tumorigenesis, metastasis, and chemotherapy resistance. The genetic profile of DAN-G includes mutations in key oncogenes and tumor suppressor genes, which are characteristic of pancreatic adenocarcinomas. This makes the cell line a valuable model for understanding the molecular mechanisms underlying pancreatic cancer and for testing new therapeutic strategies. In addition to its applications in cancer research, the DAN-G cell line has been used to study the cellular processes involved in the progression of pancreatic ductal adenocarcinoma, including cell cycle regulation, apoptosis, and signal transduction pathways. The cells exhibit aggressive in vitro growth characteristics and have the ability to form tumors in immunocompromised mice, which simulates the human disease and provides an in vivo system for evaluating the efficacy of anticancer drugs. Researchers also employ this cell line to investigate the role of the tumor microenvironment in pancreatic cancer progression and resistance to therapy.
生物體	Human
組織	Pancreas
疾病	Adenocarcinoma
同義詞	Dan-G, DanG, DANG

年齡	68 years
性別	Female
形態學	Epithelial-like
生長特性	Adherent

引用	DAN-G (Cytion catalog number 300162)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0243

蛋白質表達	P53 negative
致瘤性	Yes, in nude mice
突變譜	DAN-G cells carry a homozygous Kras mutation in codon12: GGT(Gly) >GTT(Val)

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
倍增時間	33 hours
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	3 to 4 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days
流體更新	2 to 3 times per week
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300162-131123	分析證明書	23. May. 2025	300162
300162-260525	分析證明書	21. Jul. 2025	300162

DAN-G Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)