Colo-320DM Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300153

安全資料表

產品說明書

分析證明書 (CoA)

說明	The COLO-320DM cell line is a human colorectal adenocarcinoma cell line established from the metastatic site of a 55-year-old Caucasian female. This cell line exhibits unique characteristics that are significant for the study of colorectal cancer metastasis and the effects of chemotherapeutic agents. It is noteworthy for its high expression of carcinoembryonic antigen (CEA), a valuable biomarker used in the monitoring and diagnosis of colorectal cancer. COLO-320DM cells are adherent with an epithelial-like morphology. They are often used in research focused on the cellular and molecular mechanisms underlying colorectal cancer progression and metastasis. In addition, due to their consistent growth patterns and genetic stability over passages, they serve as a reliable model for in vitro experiments investigating cancer cell biology, drug response, and gene expression related to colorectal cancer. These cells also present a particular interest for genetic studies, especially those related to the pathways involved in metastasis and response to chemotherapy. Researchers utilize COLO-320DM to explore signaling pathways, cellular response to hypoxia, and interactions between cancer cells and the tumor microenvironment. The cell line has been instrumental in the development of therapeutic strategies targeting metastatic mechanisms specific to colorectal carcinoma.
生物體	Human
組織	Colon, Dukes' type C
疾病	Colorectal adenocarcinoma
同義詞	COLO_320DM, COLO-320-DM, COLO #320DM, COLO320/DM, COLO320-DM, COLO320DM, Colo320DM, COLO320 DM, COLO 320 DM, COLO 320 (DM), Colorado 320 Double Minutes

年齡	55 years
性別	Female
族裔	Caucasian
形態學	Rounded and refractile
生長特性	Adherent

引用	COLO-320DM (Cytion catalog number 300153)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0219

同工酶	PGM1,1, PGM3, 2, G6PD, B, PEP-D, 1, 6PGD, A, ES-D, 1
致瘤性	Yes, in nude mice
產品	Serotonin, norepinephrine, epinephrine, adrenocorticotropic hormone (ACTH), parathyroid hormone

培養基	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm²
流體更新	Every 3 to 5 days
解凍後的恢復	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300153-514SF	分析證明書	23. May. 2025	300153
300153-131125	分析證明書	08. Jan. 2026	300153

Colo-320DM Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)