CESS Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300262

安全資料表

產品說明書

分析證明書 (CoA)

說明	The CESS cell line is a B lymphoblastoid cell line derived from a human patient with leukemia. This cell line is commonly used to study immunoglobulin production, particularly IgG secretion, due to its strong response to cytokine stimulation. CESS cells are EBV-transformed and exhibit surface markers characteristic of mature B cells, such as CD19 and CD38. They express the sIgG1 class of immunoglobulins and serve as a model for studying B cell differentiation and function, including immune responses regulated by cytokines like interleukin-6 (IL-6), also known as B-cell stimulation factor 2 (BSF-2). IL-6 plays a crucial role in stimulating immunoglobulin production in CESS cells, making them a valuable model for investigating B cell responses in immunological research. Additionally, CESS cells have been instrumental in studies focusing on cell signaling and apoptosis. Notably, these cells have been shown to produce and respond to Nerve Growth Factor (NGF) through an autocrine signaling mechanism, expressing both high- and low-affinity NGF receptors. Blocking NGF signaling with antibodies or specific inhibitors induces apoptosis in CESS cells, characterized by Bcl-2 phosphorylation and activation of the p38 MAPK pathway. This makes CESS cells an important model for understanding the molecular mechanisms of B cell survival and apoptosis, particularly in the context of NGF signaling and its regulation of the Bcl-2 family proteins.
生物體	Human
組織	Peripheral blood
疾病	Acute myeloid leukemia
應用	Establishment of human T hybridoma cell lines
同義詞	Cess

性別	Male
族裔	European
形態學	Lymphoblast
生長特性	Suspension

引用	CESS (Cytion catalog number 300262)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0209

病毒	Transformed by EBV
產品	IL-2 after induction with TRF (T cell-replacing factor)

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 x 10⁴ cells/cm² is recommended
流體更新	2 to 3 times per week
解凍後的恢復	Allow the cells to recover from the freezing process for at least 48 hours.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300262-060526	分析證明書	24. Jun. 2026	300262

CESS Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)