BFTC-905 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$700.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305749

安全資料表

產品說明書

分析證明書 (CoA)

說明	The BFTC-905 cell line is a human transitional cell carcinoma (TCC) model derived from a high-grade, papillary bladder tumor in a female patient. It was established to represent aggressive bladder cancer and has been used in cytogenetic and molecular profiling studies to understand bladder tumor biology and therapeutic vulnerabilities. BFTC-905 exhibits a highly complex and rearranged karyotype, which includes multiple chromosomal abnormalities typical of advanced bladder cancers. These include non-random alterations such as deletions of 8p, duplications of 8q, and gains in chromosomes 7 and 20, features often linked to disease progression and poor prognosis in urothelial carcinoma. Extensive characterization using multi-color fluorescence in situ hybridization (M-FISH) has revealed numerous structural rearrangements in BFTC-905, including interchromosomal translocations and deletions affecting loci with potential relevance to tumor suppressor loss. Specifically, BFTC-905 displays a deletion of chromosome 8p21, a region frequently lost in aggressive TCC and associated with tumor suppressor genes. This cytogenetic complexity provides a valuable opportunity for dissecting gene function in the context of genomic instability, a hallmark of late-stage bladder cancers. BFTC-905 has also been included in large-scale pharmacogenomic studies such as the Cancer Cell Line Encyclopedia (CCLE) and Genomics of Drug Sensitivity in Cancer (GDSC). These resources have confirmed the molecular fidelity of BFTC-905 to primary bladder tumors and have enabled its use in predictive modeling of anticancer drug responses. Its multi-omics profile-including gene expression, mutation status, copy number variation, and DNA methylation-makes it a powerful model for investigating bladder cancer-specific therapeutic targets and resistance mechanisms.
生物體	Human
組織	Urinary bladder
疾病	Bladder carcinoma
同義詞	BFTC 905, BFTC905, Black Foot disease Transitional Carcinoma 905

年齡	51 years
性別	Female
族裔	Chinese
形態學	Epithelial
細胞類型	Epithelial
生長特性	Adherent

引用	BFTC-905 (Cytion catalog number 305749)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1083

同工酶	G6PD; MD; LD
病毒	Reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
突變譜	Mutation: NRAS, Simple, p.Gln61Leu (c.182A>T), Heterozygous (Cosmic-CLP=910926), TP53, Simple, c.673-2A>T (IVS6-2A>T), Homozygous, Note=Splice acceptor mutation (Cosmic-CLP=910926)

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
倍增時間	60-70 hours
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
播種密度	1 to 3 x 10⁴ cells/cm²
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305749-070426	分析證明書	15. May. 2026	305749

BFTC-905 Cells

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生物分子資料

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品質控制與分子分析

分析證明書 (CoA)